Gamma-ray irradiation modulates PGRMC1 expression and the number of CD56+ and FoxP3+ cells in the tumor microenvironment of endometrial endometrioid adenocarcinoma.

Autor: Zinovkin DA; Department of Pathology, Gomel State Medical University, Gomel, Belarus., Lyzikova YA; Department of Obstetrics and Gynecology, Gomel State Medical University, Gomel, Belarus., Nadyrov EA; Department of Histology, Cytology and Embryology, Gomel State Medical University, Gomel, Belarus., Petrenyov DR; University Research Laboratory, Gomel State Medical University, Gomel, Belarus., Yuzugulen J; Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus., Pranjol MZI; School of Life Sciences, University of Sussex, Brighton, UK.
Jazyk: angličtina
Zdroj: Radiation oncology journal [Radiat Oncol J] 2021 Dec; Vol. 39 (4), pp. 324-333. Date of Electronic Publication: 2021 Aug 17.
DOI: 10.3857/roj.2021.00472
Abstrakt: Purpose: Although the conventional gamma ray brachytherapy has been successful in treating endometrioid endometrial adenocarcinoma (EC), the molecular and cellular mechanisms of this anti-tumorigenic response remain unclear. Therefore, we investigated whether gamma ray irradiation induces changes in the number of FoxP3+ T-regulatory lymphocytes (Tregs), CD56+ natural killer cells (NK), and the expression of progesterone receptor membrane component 1 (PGRMC1) in the tumor microenvironment (TME).
Materials and Methods: According to the inclusion criteria, 127 cases were selected and grouped into irradiation-treated (Rad+) and control (underwent surgery) groups and analyzed using immunohistochemistry. Predictive prognostic values were analyzed using Mann-Whitney U test, ROC analysis, relative risk, log-rank, Spearman rank tests and multivariate Cox's regression.
Results: We observed significant differences (p < 0.001) between the radiation-treated patients and the control groups in FoxP3+ Tregs numbers, CD56+ NK cells and PGRMC1 expression. Gamma ray induced a 3.71- and 3.39-fold increase in the infiltration of FoxP3+ cells, CD56+ NK cells, respectively and 0.0034-fold change in PGRMC1 expression. Univariate and multivariate analyses revealed predictive role of the parameters. In the irradiated patients' group, inverted correlations between clinical unfavorable outcome, FoxP3+ Tregs and CD56+ NK cells were observed.
Conclusion: Our results suggest an immune-modulating role, specifically by increasing immune cell infiltration, of gamma radiation in the TME which may potentially be utilized as biomarkers in prognostic values.
Databáze: MEDLINE
Externí odkaz: