Research on the mechanisms of taraxerol for the treatment of gastric cancer effect based on network pharmacology.

Autor:	Huo B; Department of Traditional Chinese Medicine, 117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P. R. China., Song Y; Department of Traditional Chinese Medicine, 117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P. R. China., Tan B; Department of General Surgery, 117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P. R. China., Li J; Department of Traditional Chinese Medicine, 117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P. R. China., Zhang J; Department of Traditional Chinese Medicine, 117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P. R. China., Zhang F; Department of Gastroenterology Pharmacology, 117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P. R. China., Chang L; HeBei University of Chinese Medicine, No. 3 Xing yuan Road, Lu quan District, Shijiazhuang, Hebei 050200, P. R. China.
Jazyk:	angličtina
Zdroj:	International journal of immunopathology and pharmacology [Int J Immunopathol Pharmacol] 2022 Jan-Dec; Vol. 36, pp. 20587384211063962.
DOI:	10.1177/20587384211063962
Abstrakt:	Background: Modern pharmacological studies have shown that traditional Chinese medicine (TCM) Taraxacum mongolicum possesses anti-cancer activity. Taraxerol (TRX) is a pentacyclic triterpene isolated from T. mongolicum , which is widely used in clinical treatment, and its anti-cancer effects have been extensively studied. However, the effects and molecular mechanism of TRX in gastric cancer (GC) have not been fully explicated. Methods: We used public databases to derive information on potential targets of TRX and proteins related to GC. Also, STRING and R3.6.2 software were used to analyze the protein-protein interaction (PPI). The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were done to explain the potential mechanism underlying the regulatory role of TRX in GC. The role of TRX in GC was verified by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay, apoptosis analysis, Transwell assay, and wound healing assay, and the key signaling pathways were verified. Results: We identified 135 potential targets for the treatment of GC via network pharmacological analysis. GO and KEGG enrichment analysis showed that steroid hormone receptor activity and the PI3K/AKT signaling pathway were the biological processes and pathways with the highest degree of enrichment. Additionally, cellular experiments revealed that TRX inhibited the proliferation, migration, and invasion of GC cells as well as induced G1 phase arrest and apoptosis in GC cells. Conclusion: Here, we used multi-target and multi-pathway network pharmacological analysis to verify the anti-cancer activity of TRX in GC. Also, in vitro experimental data were used to derive the potential molecular mechanism.
Databáze:	MEDLINE
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