Target site mutations underlying insecticide resistance in Tunisian populations of Myzus persicae (Sulzer) on peach orchards and potato crops.

Autor: Hlaoui A; Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, INRAT, Université de Carthage, Ariana, Tunisie.; Département Santé Végétale et Environnement, Institut National Agronomique de Tunisie, INAT, Université de Carthage, Tunis, Tunisie., Chiesa O; Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Piacenza, Italy., Figueroa CC; Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Talca, Chile.; Instituto de Ciencias Biológicas, Universidad de Talca, Talca, Chile., Souissi R; Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, INRAT, Université de Carthage, Ariana, Tunisie., Mazzoni E; Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Piacenza, Italy., Boukhris-Bouhachem S; Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, INRAT, Université de Carthage, Ariana, Tunisie.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2022 Apr; Vol. 78 (4), pp. 1594-1604. Date of Electronic Publication: 2022 Jan 25.
DOI: 10.1002/ps.6778
Abstrakt: Background: The massive use of synthetic insecticides strongly affects the level of insecticide resistance in populations of Myzus persicae worldwide. The selection of target site insensitivity-mutations is particularly worrying in areas where agro-industrial crops are vulnerable to the attacks of aphids that vector viruses, as in the case of Tunisia. Knowledge of the resistance mechanisms evolved locally in this aphid pest is a prerequisite to improving and retaining the sustainability of integrated pest management strategies.
Results: Target site mutations were surveyed in several populations of M. persicae collected from peach and potato crops between 2011 and 2017 in three Tunisian regions using real-time allele-specific PCR. The L1014F mutation (kdr locus) was found at a moderate frequency mostly in the heterozygous state and the homozygous resistant genotype was very uncommon. The M918T mutation (super-kdr locus) was present in a few heterozygous individuals, whereas the M918L mutation was detected for the first time in Tunisia and extreme North Africa. This latter mutation was shown to be widespread and well-established in Tunisia mainly as homozygous individuals, and was more abundant on peach than on potato crops. The S431F mutation (MACE) was found in a few heterozygous individuals. No individuals carrying the R81T mutation linked to neonicotinoid resistance were detected.
Conclusion: This study points out a critical situation for the efficacy of pyrethroid insecticides to control M. persicae populations in Tunisia. It also confirms the rapid spread of the M918L mutation which has been detected in many different areas of the Mediterranean basin. © 2022 Society of Chemical Industry.
(© 2022 Society of Chemical Industry.)
Databáze: MEDLINE
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