Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria.

Autor: Madigan MT; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA., Absher JN; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA., Mayers JE; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA., Asao M; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA., Jung DO; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA., Bender KS; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL, 62901, USA., Kempher ML; Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, 73019, USA., Hayward MK; Division of Natural Sciences, Indiana Wesleyan University, Marion, IN, 46953, USA., Sanguedolce SA; Division of Natural Sciences, Indiana Wesleyan University, Marion, IN, 46953, USA., Brown AC; Division of Natural Sciences, Indiana Wesleyan University, Marion, IN, 46953, USA., Takaichi S; Department of Molecular Microbiology, Tokyo University of Agriculture, Tokyo, 156-8502, Japan., Kurokawa K; Advanced Genomics Center, National Institute of Genetics, Shizuoka, Japan., Toyoda A; Advanced Genomics Center, National Institute of Genetics, Shizuoka, Japan., Mori H; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Kanagawa, Japan., Tsukatani Y; Advanced Genomics Center, National Institute of Genetics, Shizuoka, Japan., Wang-Otomo ZY; Faculty of Science, Ibaraki University, Mito, Japan., Ward DM; Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT, 59717, USA., Sattley WM; Division of Natural Sciences, Indiana Wesleyan University, Marion, IN, 46953, USA. matthew.sattley@indwes.edu.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Jan 04; Vol. 204 (1), pp. 115. Date of Electronic Publication: 2022 Jan 04.
DOI: 10.1007/s00203-021-02715-7
Abstrakt: We describe a new species of purple sulfur bacteria (Chromatiaceae, anoxygenic phototrophic bacteria) isolated from a microbial mat in the sulfidic geothermal outflow of a hot spring in Rotorua, New Zealand. This phototroph, designated as strain NZ, grew optimally near 45 °C but did not show an absorption maximum at 915 nm for the light-harvesting-reaction center core complex (LH1-RC) characteristic of other thermophilic purple sulfur bacteria. Strain NZ had a similar carotenoid composition as Thermochromatium tepidum, but unlike Tch. tepidum, grew photoheterotrophically on acetate in the absence of sulfide and metabolized thiosulfate. The genome of strain NZ was significantly larger than that of Tch. tepidum but slightly smaller than that of Allochromatium vinosum. Strain NZ was phylogenetically more closely related to mesophilic purple sulfur bacteria of the genus Allochromatium than to Tch. tepidum. This conclusion was reached from phylogenetic analyses of strain NZ genes encoding 16S rRNA and the photosynthetic functional gene pufM, from phylogenetic analyses of entire genomes, and from a phylogenetic tree constructed from the concatenated sequence of 1090 orthologous proteins. Moreover, average nucleotide identities and digital DNA:DNA hybridizations of the strain NZ genome against those of related species of Chromatiaceae supported the phylogenetic analyses. From this collection of properties, we describe strain NZ here as the first thermophilic species of the genus Allochromatium, Allochromatium tepidum NZ T , sp. nov.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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