Native Mass Spectrometry: Recent Progress and Remaining Challenges.

Autor:	Karch KR; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA; email: wysocki.11@osu.edu.; Resource for Native Mass Spectrometry Guided Structural Biology, The Ohio State University, Columbus, Ohio, USA., Snyder DT; Resource for Native Mass Spectrometry Guided Structural Biology, The Ohio State University, Columbus, Ohio, USA., Harvey SR; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA; email: wysocki.11@osu.edu.; Resource for Native Mass Spectrometry Guided Structural Biology, The Ohio State University, Columbus, Ohio, USA., Wysocki VH; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA; email: wysocki.11@osu.edu.; Resource for Native Mass Spectrometry Guided Structural Biology, The Ohio State University, Columbus, Ohio, USA.
Jazyk:	angličtina
Zdroj:	Annual review of biophysics [Annu Rev Biophys] 2022 May 09; Vol. 51, pp. 157-179. Date of Electronic Publication: 2022 Jan 04.
DOI:	10.1146/annurev-biophys-092721-085421
Abstrakt:	Native mass spectrometry (nMS) has emerged as an important tool in studying the structure and function of macromolecules and their complexes in the gas phase. In this review, we cover recent advances in nMS and related techniques including sample preparation, instrumentation, activation methods, and data analysis software. These advances have enabled nMS-based techniques to address a variety of challenging questions in structural biology. The second half of this review highlights recent applications of these technologies and surveys the classes of complexes that can be studied with nMS. Complementarity of nMS to existing structural biology techniques and current challenges in nMS are also addressed.
Databáze:	MEDLINE
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