Non-invasive longitudinal imaging of VEGF-induced microvascular alterations in skin wounds.

Autor:	Liu YH; Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich, Switzerland., Brunner LM; Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland., Rebling J; Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich, Switzerland., Ben-Yehuda Greenwald M; Institute of Molecular Health Sciences, Department of Biology, ETH Zurich, Zurich, Switzerland., Werner S; Institute of Molecular Health Sciences, Department of Biology, ETH Zurich, Zurich, Switzerland., Detmar M; Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland., Razansky D; Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich, Switzerland.
Jazyk:	angličtina
Zdroj:	Theranostics [Theranostics] 2022 Jan 01; Vol. 12 (2), pp. 558-573. Date of Electronic Publication: 2022 Jan 01 (Print Publication: 2022).
DOI:	10.7150/thno.65287
Abstrakt:	Background: Microcirculation is essential for skin homeostasis and repair. A variety of growth factors have been identified as important regulators of wound healing. However, direct observation and longitudinal monitoring of skin remodeling in an unperturbed in vivo environment remains challenging. Methods: We report on non-invasive longitudinal imaging of the wound healing process in transgenic mice overexpressing vascular endothelial growth factor A (VEGF-A) in keratinocytes by means of large-scale optoacoustic microscopy (LSOM). This rapid, label-free, high throughput intravital microscopy method averts the use of dorsal skin-fold chambers, allowing for fully non-invasive repeated imaging of intact wounds with capillary resolution over field-of-view spanning several centimeters. Results: We observed VEGF-driven enhancement of dermal vascularization in ears, dorsal skin and healing wounds and quantified the hemoglobin content, fill fraction, vessel diameter and tortuosity. The in vivo findings were further corroborated by detailed side-by-side classical histological whole-mount vascular stainings and pan-endothelial CD31 immunofluorescence. Conclusion: The new approach is suitable for supplementing or replacing the cumbersome histological procedures in a broad range of skin regeneration and tissue engineering applications. Competing Interests: Competing Interests: The authors have declared that no competing interest exists. (© The author(s).)
Databáze:	MEDLINE
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