Adaptation and validation of a method for evaluating the bactericidal activity of ethyl alcohol in gel format 70% (w/w).

Autor: Cruz AFD; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil. Electronic address: amanda.fermiano@hotmail.com., Abreu AO; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil., Souza PA; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil., Deveza B; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil., Medeiros CT; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil., Sousa VS; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil., Sabagh BP; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil., Villas Bôas MHS; Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2022 Feb; Vol. 193, pp. 106402. Date of Electronic Publication: 2021 Dec 30.
DOI: 10.1016/j.mimet.2021.106402
Abstrakt: Ethyl alcohol 70% (w/w) is a broad-spectrum bactericidal agent that is mandatorily marketed as a gel in Brazil since 2002. However, there is no method described for the microbiological quality control of surface disinfectants in the form of gel, which makes it impossible to monitor the effectiveness of these products by the Health Surveillance. Thus, the present study aimed to adapt an analytical method and validate it so that it is possible to evaluate the bactericidal activity of ethyl alcohol-based disinfectants at 70% (w/w) in gel form. The proposed analytical method is an adaptation of a method developed by the Association of Official Analytical Chemists (AOAC), which is currently used to evaluate the bacterial activity of disinfectants in the form of spray and aerosol. The method consisted of challenging the disinfectant by putting it in contact with 60 carriers - previously contaminated with the test microorganism - during the contact time established by the manufacturer. The bacteria used in this work were Staphylococcus aureus CBRVS 00039 ATCC 6538, Salmonella enterica CBRVS 00028 ATCC 10708, and Pseudomonas aeruginosa CBRVS 00025 ATCC 15442. The parameters chosen for the validation of the method were: matrix effect, robustness, and repeatability. The experiments performed with a disinfectant matrix showed that there is no interference of the gel on the effectiveness of alcohol. Satisfactory results regarding the evaluation of bactericidal activity were obtained for the three microorganisms tested when the volume of 100 μL of disinfectant was applied. The method also showed good repeatability, as it proved to be robust with modification of incubation times, equipment and analysts. The development and validation of this method were extremely important for the quality monitoring of these products, besides being the first method described for the evaluation of gel disinfectants.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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