Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells.

Autor:	Daum JR; Cell Cycle and Cancer Biology Research program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA., DuBose CO; Cell Cycle and Cancer Biology Research program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA., Sivakumar S; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Gorbsky GJ; Cell Cycle and Cancer Biology Research program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. GJG@omrf.org.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2415, pp. 61-86.
DOI:	10.1007/978-1-0716-1904-9_5
Abstrakt:	Live-cell fluorescence microscopy is an effective tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors and after RNA interference-mediated or CRISPR knockout-mediated depletion of protein targets. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. Herein are strategies to monitor and analyze mammalian cell mitosis utilizing either a wide field or a light sheet, inverted, fluorescence microscope. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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