Blastocyst development after fertilization with in vitro spermatids derived from nonhuman primate embryonic stem cells.

Autor:	Khampang S; Division of Neuropharmacology and Neurologic Diseases; Yerkes National Primate Research Center; Atlanta, Georgia.; Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima, Thailand., Cho IK; Division of Neuropharmacology and Neurologic Diseases; Yerkes National Primate Research Center; Atlanta, Georgia.; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Punyawai K; Division of Neuropharmacology and Neurologic Diseases; Yerkes National Primate Research Center; Atlanta, Georgia., Gill B; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Langmo JN; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Nath S; Department of Genetics, University of Georgia, Athens, Georgia., Greeson KW; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Symosko KM; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Fowler KL; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Tian S; Division of Neuropharmacology and Neurologic Diseases; Yerkes National Primate Research Center; Atlanta, Georgia., Statz JP; Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon.; Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon., Steves AN; Division of Neuropharmacology and Neurologic Diseases; Yerkes National Primate Research Center; Atlanta, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia., Parnpai R; Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima, Thailand., White MA; Department of Genetics, University of Georgia, Athens, Georgia., Hennebold JD; Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon.; Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon., Orwig KE; Magee-Womens Research Institute and Departments of Obstetrics, Gynecology, and Reproductive Sciences, Cell Biology and Bioengineering; University of Pittsburgh; Pittsburgh, Pennsylvania., Simerly CR; Magee-Womens Research Institute and Departments of Obstetrics, Gynecology, and Reproductive Sciences, Cell Biology and Bioengineering; University of Pittsburgh; Pittsburgh, Pennsylvania., Schatten G; Magee-Womens Research Institute and Departments of Obstetrics, Gynecology, and Reproductive Sciences, Cell Biology and Bioengineering; University of Pittsburgh; Pittsburgh, Pennsylvania., Easley CA 4th; Division of Neuropharmacology and Neurologic Diseases; Yerkes National Primate Research Center; Atlanta, Georgia.; Department of Environmental Health Science, College of Public Health, University of Georgia; Athens, Georgia.; Regenerative Bioscience Center; University of Georgia; Athens, Georgia.
Jazyk:	angličtina
Zdroj:	F&S science [F S Sci] 2021 Nov; Vol. 2 (4), pp. 365-375. Date of Electronic Publication: 2021 Sep 08.
DOI:	10.1016/j.xfss.2021.09.001
Abstrakt:	Objective: To demonstrate that functional spermatids can be derived in vitro from nonhuman primate pluripotent stem cells. Design: Green fluorescent protein-labeled, rhesus macaque nonhuman primate embryonic stem cells (nhpESCs) were differentiated into advanced male germ cell lineages using a modified serum-free spermatogonial stem cell culture medium. In vitro-derived round spermatid-like cells (rSLCs) from differentiated nhpESCs were assessed for their ability to fertilize rhesus oocytes by intracytoplasmic sperm(atid) injection. Setting: Multiple academic laboratory settings. Patients: Not applicable. Interventions: Intracytoplasmic sperm(atid) injection of in vitro-derived spermatids from nhpESCs into rhesus macaque oocytes. Main Outcome Measures: Differentiation into spermatogenic cell lineages was measured through multiple assessments including ribonucleic acid sequencing and immunocytochemistry for various spermatogenic markers. In vitro spermatids were assessed for their ability to fertilize oocytes by intracytoplasmic sperm(atid) injection by assessing early fertilization events such as spermatid deoxyribonucleic acid decondensation and pronucleus formation/apposition. Preimplantation embryo development from the one-cell zygote stage to the blastocyst stage was also assessed. Results: Nonhuman primate embryonic stem cells can be differentiated into advanced germ cell lineages, including haploid rSLCs. These rSLCs undergo deoxyribonucleic acid decondensation and pronucleus formation/apposition when microinjected into rhesus macaque mature oocytes, which, after artificial activation and coinjection of ten-eleven translocation 3 protein, undergo embryonic divisions with approximately 12% developing successfully into expanded blastocysts. Conclusions: This work demonstrates that rSLCs, generated in vitro from primate pluripotent stem cells, mimic many of the capabilities of in vivo round spermatids and perform events essential for preimplantation development. To our knowledge, this work represents, for the first time, that functional spermatid-like cells can be derived in vitro from primate pluripotent stem cells.
Databáze:	MEDLINE
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