Autor: |
Añaña DC; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Waller SB; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Giordani C; Faculdade de Medicina Veterinária, Universidade de Caxias do Sul, Caxias do Sul, RS, Brasil., Perera SC; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil., de Almeida Capella G; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Berne N; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Strothmann AL; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Freitag RA; Departamento de Química Orgânica, Instituto de Química e Geociências, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Cleff MB; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil. |
Abstrakt: |
This study aimed to evaluate the ovicidal activity of the hydroalcoholic extract of Schinus terebinthifolia (SCH; T1) against Ancylostoma spp. and its influence of storage time in the extract stored for 36 months (T36). Eggs of Ancylostoma spp. were obtained from naturally parasitized dogs, and used for the larval hatchability test, where the eggs were exposed to T1 and T36 extracts of SCH (15-0.625 mg/mL). In T1, all concentrations inhibited more than 80% of the eggs, being 100% at concentrations between 15 and 5 mg/mL ( p > 0.05). At T36, all concentrations were active, even the ones between 2.5 and 0.625 mg/mL, with 100% inhibition ( p < 0.05), revealing that the storage time maintained the ovicidal action. By LC-MS, T36 presented ethyl gallate, myricitrin, and gallic acid as major compounds. These findings support the promising use of SCH extract as an ovicide against Ancylostoma spp., even stored for 36 months of shelf life. |