First transcriptome of the copepod Gladioferens pectinatus subjected to chronic contaminant exposures.

Autor: Barrick A; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand. Electronic address: Andrew.barrick@cawthron.org.nz., Laroche O; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand., Boundy M; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand., Pearman JK; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand., Wiles T; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand., Butler J; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand., Pochon X; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand; Institute of Marine Science, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand., Smith KF; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand; School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand., Tremblay LA; Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand; School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2022 Feb; Vol. 243, pp. 106069. Date of Electronic Publication: 2021 Dec 24.
DOI: 10.1016/j.aquatox.2021.106069
Abstrakt: Contaminants are often at low concentrations in ecosystems and their effects on exposed organisms can occur over long periods of time and across multiple generations. Alterations to subcellular mechanistic pathways in response to exposure to contaminants can provide insights into mechanisms of toxicity that methods measuring higher levels of biological may miss. Analysis of the whole transcriptome can identify novel mechanisms of action leading to impacts in exposed biota. The aim of this study was to characterise how exposures to copper, benzophenone and diclofenac across multiple generations altered molecular expression pathways in the marine copepod Gladioferens pectinatus. Results of the study demonstrated differential gene expression was observed in cultures exposure to diclofenac (569), copper (449) and benzophenone (59). Pathways linked to stress, growth, cellular and metabolic processes were altered by exposure to all three contaminants with genes associated with oxidative stress and xenobiotic regulation also impacted. Protein kinase functioning, cytochrome P450, transcription, skeletal muscle contraction/relaxation, mitochondrial phosphate translocator, protein synthesis and mitochondrial methylation were all differentially expressed with all three chemicals. The results of the study also suggested that using dimethyl sulfoxide as a dispersant influenced the transcriptome and future research may want to investigate it's use in molecular studies. Data generated in this study provides a first look at transcriptomic response of G. pectinatus exposed to contaminants across multiple generations, future research is needed to validate the identified biomarkers and link these results to apical responses such as population growth to demonstrate the predictive capacity of molecular tools.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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