IRF8 and BATF3 interaction enhances the cDC1 specific Pfkfb3 gene expression.

Autor: Chauhan KS; Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, India; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA(1)., Das A; Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, India., Jaiswal H; Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, India; Laboratory of Molecular Immunology, National Institute of Allergy and, Infectious Diseases, National Institutes of Health, Bethesda, MD, USA(2)., Saha I; Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, India; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA(3)., Kaushik M; Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, India; School of Biotechnology, Jawaharlal Nehru University, New Delhi, India(4)., Patel VK; Cell Biology Laboratory, National Institute of Immunology, New Delhi, India., Tailor P; Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, India; Special Centre for Systems Medicine (SCSM), Jawaharlal Nehru University, New Delhi, India. Electronic address: tailorp@nii.ac.in.
Jazyk: angličtina
Zdroj: Cellular immunology [Cell Immunol] 2022 Jan; Vol. 371, pp. 104468. Date of Electronic Publication: 2021 Dec 10.
DOI: 10.1016/j.cellimm.2021.104468
Abstrakt: Dendritic cells (DCs) play central role in innate as well as adaptive immune responses regulated by diverse DC subtypes that vary in terms of surface markers, transcriptional profile and functional responses. Generation of DC diversity from progenitor stage is tightly regulated by complex molecular inter-play between transcription factors. We earlier demonstrated that Batf3 and Id2 expression have a synergistic effect on the Irf8 directed classical cDC1 development. In present study, Bi-molecular fluorescence complementation assay suggested that IRF8 interacts with BATF3, and ID2 may aid cDC1 development independently. Genome wide recruitment analysis of IRF8 and BATF3 from different DC subtypes led to identification of the overlapping regions of occupancy by these two transcription factors. Further analysis of overlapping peaks of IRF8 and BATF3 occupancy in promoter region within the cDC1 subtype specific transcriptional pattern identified a metabolically important Pfkfb3 gene. Among various immune cell types; splenic cDC1 subtype displayed enhanced expression of Pfkfb3. Analysis of Irf8 -/- , Irf8 R294C and Batf3 DCKO DC confirmed direct regulation of Pfkfb3 enhanced expression specifically in cDC1 subtype. Further we show that inhibition of PFKFB3 enzymatic activity by a chemical agent PFK15 led to reduction in cDC1 subtype in both in vitro FLDC cultures as well as in vivo mouse spleens. Together, our study identified the direct regulation of cDC1 specific enhanced expression of Pfkfb3 in glycolysis and cDC1 biology.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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