Extraction method development for nanoplastics from oyster and fish tissues.

Autor: Chang YS; Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei 10031, Taiwan., Chou SH; Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan., Jhang YJ; Institute of Analytical and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan., Wu TS; National Synchrotron Radiation Research Center (NSRRC), Hsinchu 30013, Taiwan., Lin LX; School of Food Safety, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan., Soo YL; Department of Physics, National Tsing Hua University 30013, Taiwan., Hsiao IL; Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei 10031, Taiwan; School of Food Safety, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan. Electronic address: ilunhsiao@tmu.edu.tw.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Mar 25; Vol. 814, pp. 152675. Date of Electronic Publication: 2021 Dec 28.
DOI: 10.1016/j.scitotenv.2021.152675
Abstrakt: Nanoplastics are now found in some environmental media and consumer products. However, very limited data on nanoplastics are available for one of the main human consumption sources of microplastics: seafood. Unlike microplastics, a method for extracting nanoplastics from seafood is still lacking. Herein, a combination of common extraction techniques including enzymatic digestion, sequential membrane filtration, centrifugal concentration, and purification (dialysis and sodium dodecylsulfate (SDS) incubation), was developed to extract nanoplastics from oyster and fish tissues. Corolase with subsequent lipase treatment achieved the highest digestion efficiencies (88- 89%) for non-homogenized tissues compared to other proteases and additional cellulase or H 2 O 2 treatment. With the exception of polyethylene terephthalate (PET), enzymatic digestion did not change the morphology or structure of polyvinyl chloride (PVC), polyethylene (PE), or polystyrene (PS) nanoplastic particles, and the subsequent extraction procedures had good recoveries of 71- 110% for fluorescence-labeled 76-nm PVC and 100- and 750-nm PS, as validated by a Nanoparticle Tracking Analysis (NTA). Few of the 10 11 digested residual particles of 150- 300 nm in diameter per oyster or per serving of fish tissue were left in the method blank. Consequently, this efficient approach could be used as a pretreatment protocol for current potential nanoplastic detection methods.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: