The role of basolateral amygdala orexin 1 receptors on the modulation of pain and psychosocial deficits in nitroglycerin-induced migraine model in adult male rats.

Autor: Askari-Zahabi K; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Abbasnejad M; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Kooshki R; Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran., Raoof M; Department of Orofacial Pain and Dysfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Endodontology Research Center, Kerman University of Medical Sciences, Kerman, Iran., Esmaeili-Mahani S; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Pourrahimi AM; Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran., Zamyad M; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Jazyk: angličtina
Zdroj: The Korean journal of pain [Korean J Pain] 2022 Jan 01; Vol. 35 (1), pp. 22-32.
DOI: 10.3344/kjp.2022.35.1.22
Abstrakt: Background: Migraine headaches have been associated with sensory hyperactivity and anomalies in social/emotional responses. The main objective of this study was to evaluate the potential involvement of orexin 1 receptors (Orx1R) within the basolateral amygdala (BLA) in the modulation of pain and psychosocial dysfunction in a nitroglycerin (NTG)-induced rat model of migraine.
Methods: Adult male Wistar rats were injected with NTG (5 mg/kg, intraperitoneal) every second day over nine days to induce migraine. The experiments were done in the following six groups (6 rats per group): untreated control, NTG, NTG plus vehicle, and NTG groups that were post-treated with intra-BLA microinjection of Orx1R antagonist SB-334867 (10, 20, and 40 nM). Thermal hyperalgesia was assessed using the hot plate and tail-flick tests. Moreover, the elevated plus maze (EPM) and open field (OF) tests were used to assess anxiety-like behaviors. The animals' sociability was evaluated using the three-chamber social task. The NTG-induced photophobia was assessed using a light-dark box.
Results: We observed no change in NTG-induced thermal hyperalgesia following administration of SB-334867 (10, 20, and 40 nM). However, SB-334867 (20 and 40 nM) aggravated the NTG-induced anxiogenic responses in both the EPM and OF tasks. The NTG-induced social impairment was overpowered by SB-334867 at all doses. Time spent in the dark chamber of light-dark box was significantly increased in rats treated with SB-334867 (20 and 40 nM/rat).
Conclusions: The findings suggest a role for Orx1R within the BLA in control comorbid affective complaints with migraine in rats.
Databáze: MEDLINE
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