Immunoprofiling of active and inactive systemic juvenile idiopathic arthritis reveals distinct biomarkers: a single-center study.

Autor: Qu H; Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Division of Rheumatology, Karolinska University Hospital, Stockholm, Sweden., Sundberg E; Unit of Pediatric Rheumatology, Karolinska University Hospital, Stockholm, Sweden.; Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Aulin C; Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Division of Rheumatology, Karolinska University Hospital, Stockholm, Sweden., Neog M; Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Division of Rheumatology, Karolinska University Hospital, Stockholm, Sweden., Palmblad K; Unit of Pediatric Rheumatology, Karolinska University Hospital, Stockholm, Sweden.; Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Horne AC; Unit of Pediatric Rheumatology, Karolinska University Hospital, Stockholm, Sweden.; Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Granath F; Division of Clinical Epidemiology, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden., Ek A; Center for Occupational and Environmental Medicine, Region Stockholm, Stockholm, Sweden., Melén E; Sachs Children's Hospital, Stockholm, Sweden.; Department of Clinical Sciences and Education, Karolinska Institutet, Södersjukhuset, Stockholm, Sweden., Olsson M; Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Division of Rheumatology, Karolinska University Hospital, Stockholm, Sweden., Harris HE; Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden. helena.harris@ki.se.; Division of Rheumatology, Karolinska University Hospital, Stockholm, Sweden. helena.harris@ki.se.
Jazyk: angličtina
Zdroj: Pediatric rheumatology online journal [Pediatr Rheumatol Online J] 2021 Dec 28; Vol. 19 (1), pp. 173. Date of Electronic Publication: 2021 Dec 28.
DOI: 10.1186/s12969-021-00660-9
Abstrakt: Background: This study aimed to perform an immunoprofiling of systemic juvenile idiopathic arthritis (sJIA) in order to define biomarkers of clinical use as well as reveal new immune mechanisms.
Methods: Immunoprofiling of plasma samples from a clinically well-described cohort consisting of 21 sJIA patients as well as 60 age and sex matched healthy controls, was performed by a highly sensitive proteomic immunoassay. Based on the biomarkers being significantly up- or down-regulated in cross-sectional and paired analysis, related canonical pathways and cellular functions were explored by Ingenuity Pathway Analysis (IPA).
Results: The well-studied sJIA biomarkers, IL6, IL18 and S100A12, were confirmed to be increased during active sJIA as compared to healthy controls. IL18 was the only factor found to be increased during inactive sJIA as compared to healthy controls. Novel factors, including CASP8, CCL23, CD6, CXCL1, CXCL11, CXCL5, EIF4EBP1, KITLG, MMP1, OSM, SIRT2, SULT1A1 and TNFSF11, were found to be differentially expressed in active and/or inactive sJIA and healthy controls. No significant pathway activation could be predicted based on the limited factor input to the IPA. High Mobility Group Box 1 (HMGB1), a damage associated molecular pattern being involved in a series of inflammatory diseases, was determined to be higher in active sJIA than inactive sJIA.
Conclusions: We could identify a novel set of biomarkers distinguishing active sJIA from inactive sJIA or healthy controls. Our findings enable a better understanding of the immune mechanisms active in sJIA and aid the development of future diagnostic and therapeutic strategies.
(© 2021. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje