| Autor: |
Wang Y; Department of Pathology, Johns Hopkins University, Baltimore, Maryland 21231, United States., Hu Y; Department of Pathology, Johns Hopkins University, Baltimore, Maryland 21231, United States., Höti N; Department of Pathology, Johns Hopkins University, Baltimore, Maryland 21231, United States., Huang L; Department of Physiology and Biophysics, University of California, Irvine, California 92697, United States., Zhang H; Department of Pathology, Johns Hopkins University, Baltimore, Maryland 21231, United States. |
| Abstrakt: |
Cells perform various functions by proteins via protein complexes. Characterization of protein complexes is critical to understanding their biological and clinical significance and has been one of the major efforts of functional proteomics. To date, most protein complexes are characterized by the in vitro system from protein extracts after the cells or tissues are lysed, and it has been challenging to determine which of these protein complexes are formed in intact cells. Herein, we report an approach to preserve protein complexes using in vivo cross-linking, followed by size exclusion chromatography and data-independent acquisition mass spectrometry. This approach enables the characterization of in vivo protein complexes from cells or tissues, which allows the determination of protein complexes in clinical research. More importantly, the described approach can identify protein complexes that are not detected by the in vitro system, which provide unique protein function information. |
