| Autor: |
Motolinía-Alcántara EA; Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril de San Rafael Atlixco 186, Ciudad de México 09310, Mexico., Castillo-Araiza CO; Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril de San Rafael Atlixco 186, Ciudad de México 09310, Mexico., Rodríguez-Monroy M; Centro de Desarrollo de Productos Bióticos (CEPROBI), Departamento de Biotecnología, Instituto Politécnico Nacional (IPN), Yautepec 62731, Mexico., Román-Guerrero A; Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril de San Rafael Atlixco 186, Ciudad de México 09310, Mexico., Cruz-Sosa F; Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril de San Rafael Atlixco 186, Ciudad de México 09310, Mexico. |
| Jazyk: |
angličtina |
| Zdroj: |
Plants (Basel, Switzerland) [Plants (Basel)] 2021 Dec 14; Vol. 10 (12). Date of Electronic Publication: 2021 Dec 14. |
| DOI: |
10.3390/plants10122762 |
| Abstrakt: |
The large-scale production of plant-derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds for the treatment and prevention of degenerative diseases is nowadays considered an engineering challenge due to the large number of operational factors that need to be considered during their design and scale-up. The plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as the conventional whole-plant extraction, not only for avoiding the overexploitation of plant species, but also for achieving better yields and having excellent scaling-up attributes. The selection of the bioreactor configuration depends on intrinsic cell culture properties and engineering considerations related to the effect of operating conditions on thermodynamics, kinetics, and transport phenomena, which together are essential for accomplishing the large-scale production of PDSM. To this end, this review, firstly, provides a comprehensive appraisement of PDSM, essentially those with demonstrated importance and utilization in pharmaceutical industries. Then, special attention is given to PDSM obtained out of CSC. Finally, engineering aspects related to the bioreactor configuration for CSC stating the effect of the operating conditions on kinetics and transport phenomena and, hence, on the cell viability and production of PDSM are presented accordingly. The engineering analysis of the reviewed bioreactor configurations for CSC will pave the way for future research focused on their scaling up, to produce high value-added PDSM. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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