| Autor: |
Mikhaylova E; Institute of Biochemistry and Genetics UFRC RAS, Prospekt Oktyabrya 71, 450054 Ufa, Russia., Khusnutdinov E; Institute of Biochemistry and Genetics UFRC RAS, Prospekt Oktyabrya 71, 450054 Ufa, Russia., Shein MY; Institute of Biochemistry and Genetics UFRC RAS, Prospekt Oktyabrya 71, 450054 Ufa, Russia., Alekseev VY; Institute of Biochemistry and Genetics UFRC RAS, Prospekt Oktyabrya 71, 450054 Ufa, Russia., Nikonorov Y; Institute of Biochemistry and Genetics UFRC RAS, Prospekt Oktyabrya 71, 450054 Ufa, Russia., Kuluev B; Institute of Biochemistry and Genetics UFRC RAS, Prospekt Oktyabrya 71, 450054 Ufa, Russia. |
| Abstrakt: |
Oilseed rape ( Brassica napus ) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosinolate (GSL) biosynthesis and response to stress, including fungal deceases. However, the impact of exogenous GSTF11 gene expression on resistance to powdery mildew has not yet been confirmed and requires further investigation. Transgenic B. napus was generated for this purpose. It demonstrated increased GST activity and a higher GSH:GSSG ratio under normal conditions. Powdery mildew Erysiphe crucifertaum caused 50% mortality in wild type (WT) plants. In most of transgenic plants, mycelium growth was inhibited. The infection contributed to higher GSTF11 expression and increased levels of glutathione (GSH) and oxidized glutathione (GSSG) in both transgenic and WT plants. In contrast, GSTF11 mRNA content, GST activity and GSSG level were lower only in WT plants. In transgenic plants, increased resistance to powdery mildew correlated with a lower GSH:GSSG ratio, indicating a higher content of neutralized toxic molecules. GSTF11 expression was also affected by cold stress, but not drought. At -1 °C, the expression level increased only in transgenic plants. Therefore, GSTF11 appears to be nonspecific and is able to protect plants under several types of stress. This gene could be used as a target in the production of stress tolerant cultivars. |
