Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments.

Autor: Pavletić M; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Mazor M; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Lerga M; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Mileta T; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Železnjak J; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Ružić T; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Ravlić S; Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10000 Zagreb, Croatia., Palčevski D; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia., Kirinčić J; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia., Mežnarić S; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia., Mišković A; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia., Materljan M; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia., Šustić A; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Lisnić B; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Juranić Lisnić V; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2021 Dec 02; Vol. 13 (12). Date of Electronic Publication: 2021 Dec 02.
DOI: 10.3390/v13122413
Abstrakt: During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.
Databáze: MEDLINE
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