HIV-1 Packaging Visualised by In-Gel SHAPE.

Autor: D'Souza AR; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore., Jayaraman D; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore., Long Z; Department of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK., Zeng J; Department of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK., Prestwood LJ; Department of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK., Chan C; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore., Kappei D; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.; NUS Center for Cancer Research, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Lever AML; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.; Department of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK., Kenyon JC; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.; Department of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore.; Homerton College, University of Cambridge, Cambridge CB2 8PH, UK.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2021 Nov 29; Vol. 13 (12). Date of Electronic Publication: 2021 Nov 29.
DOI: 10.3390/v13122389
Abstrakt: HIV-1 packages two copies of its gRNA into virions via an interaction with the viral structural protein Gag. Both copies and their native RNA structure are essential for virion infectivity. The precise stepwise nature of the packaging process has not been resolved. This is largely due to a prior lack of structural techniques that follow RNA structural changes within an RNA-protein complex. Here, we apply the in-gel SHAPE (selective 2'OH acylation analysed by primer extension) technique to study the initiation of HIV-1 packaging, examining the interaction between the packaging signal RNA and the Gag polyprotein, and compare it with that of the NC domain of Gag alone. Our results imply interactions between Gag and monomeric packaging signal RNA in switching the RNA conformation into a dimerisation-competent structure, and show that the Gag-dimer complex then continues to stabilise. These data provide a novel insight into how HIV-1 regulates the translation and packaging of its genome.
Databáze: MEDLINE
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