| Autor: |
Hsu AKW; Anesthesia Department, São Paulo State University (UNESP)-Botucatu, Botucatu 18618683, Brazil.; Pharmacology and Histology Department, Medical School, Integrated Regional University (URI), Erechim 99709910, Brazil., Roman SS; Pharmacology and Histology Department, Medical School, Integrated Regional University (URI), Erechim 99709910, Brazil., Bagatini MD; Academic Coordination, Biomedical Sciencies Department, Federal University of Fronteira Sul, Chapecó 89802210, Brazil., Marafon F; Postgraduate Program in Biochemistry Department, Federal University of Santa Catarina, Florianopolis 88040900, Brazil., do Nascimento Junior P; Anesthesia Department, São Paulo State University (UNESP)-Botucatu, Botucatu 18618683, Brazil., Modolo NSP; Anesthesia Department, São Paulo State University (UNESP)-Botucatu, Botucatu 18618683, Brazil. |
| Abstrakt: |
(1) Background: Intermittent fasting is a nutrition practice in which individuals fast for several hours in a day, mainly with feeding time during the daylight hours. They seek to improve metabolic performance and cellular resistance to stress. In this study, we tested the fasting protocol to investigate the glycemic effect in a laparotomy perioperative period in diabetic rats and histopathologic findings. (2) Methods: The animals were diabetic-induced with alloxan. Two groups were set according to the feeding protocol: free food and intermittent fasting, whose rats could only eat 8 h in the daylight. Both groups were anesthetized, and a laparotomy was performed. We evaluated the glucose levels during the perioperative period, and we accessed organ histology seeking damage of kidney, bowel and liver after surgical trauma, and we evaluated the wound healing process. (3) Results: Glycemic levels were improved in the intermittent fasting group, especially in the post-operative period after laparotomy. Comparing both groups' tubular damage showed interdependency with mice with worse glycemic level (Z = 2.3; p = 0.0215) and wound-healing parameters showed interdependency with rats with better glycemic status for neovascularization (Z = 2.2; p = 0.0273) and the presence of sebaceous and sweat gland in the healing process (Z = 2.30; p = 0.0215). (4) Conclusions: Intermittent fasting before surgery can be a tool to improve glycemic levels in diabetic rats, with improvement especially in the post-operative period. |
