Possible Role of Accessory Proteins in the Viral Replication for the 20I/501Y.V1 (B.1.1.7) SARS CoV-2 Variant.

Autor:	Nyayanit DA; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Sarkale P; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Shete-Aich A; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Kumar A; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Patil S; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Majumdar T; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Baradkar S; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Gawande P; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Mohandas S; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India., Yadav PD; Indian Council of Medical Research-National Institute of Virology, Pune 411021, India.
Jazyk:	angličtina
Zdroj:	Pathogens (Basel, Switzerland) [Pathogens] 2021 Dec 07; Vol. 10 (12). Date of Electronic Publication: 2021 Dec 07.
DOI:	10.3390/pathogens10121586
Abstrakt:	The emergence of new severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) has been a global concern. The B.1.1.7 variant of SARS CoV-2 is reported to cause higher transmission. The study investigates the replication cycle and transcriptional pattern of the B.1.1.7 to hypothesis the possible role of different genes in viral replication. It was observed that the B.1.1.7 variant required a longer maturation time. The transcriptional response demonstrated higher expression of ORF6 and ORF8 compared to nucleocapsid transcript till the eclipse period which might influence higher viral replication. The number of infectious viruses titer is higher in the B.1.1.7, despite a lesser copy number than B.1, indicating higher transmissibility. The experimental evidence published linked ORF6 and ORF8 to play important role in replication and we also observed their higher expression. This leads us to hypothesis the possible role of ORF6 and ORF8 in B.1.1.7 higher replication which causes higher transmission.
Databáze:	MEDLINE
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