Genetic diversity among genotypes of Uncaria guianensis (Aubl.) J.F. Gmel. maintained in an in vitro germplasm bank.

Autor: Pinto GHT; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil., Lopes AA; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil., de Freitas Morel LJ; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil., Crevelin EJ; Faculdade de Filosofia, Ciências E Letras de Ribeirão Preto, Departamento de Química, Universidade de São Paulo-USP, Ribeirão Preto, São Paulo, 14049-900 Brazil., Miranda CES; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil., Contini SHT; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil., de Castro França S; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil., Bertoni BW; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil.; Jardim Botânico de Plantas Medicinais Ordem E Progresso-JOP, Jardinópolis, São Paulo, 14680-000 Brazil., Pereira AMS; Departamento de Biotecnologia Em Plantas Medicinais, Universidade de Ribeirão (UNAERP), Ribeirão Preto, São Paulo, 14096-900 Brazil.; Jardim Botânico de Plantas Medicinais Ordem E Progresso-JOP, Jardinópolis, São Paulo, 14680-000 Brazil.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2022 Jan; Vol. 12 (1), pp. 8. Date of Electronic Publication: 2021 Dec 07.
DOI: 10.1007/s13205-021-03016-y
Abstrakt: Phytotherapeutic preparations from Uncaria guianensis (Aubl.) J.F. Gmel. (Rubiaceae) are marketed worldwide and are mainly used for their anti-inflammatory activity. The species has not yet been domesticated and is threatened by deforestation and overexploitation. It is, therefore, important to preserve and manage this genetic resource in germplasm banks, so that the extractive provision of plant material can be replaced by cultivated production. The aim of this study was to evaluate the genetic diversity among 20 genotypes maintained under in vitro conditions using 9 primers start codon targeted (SCoT) polymorphism, and to determine the concentrations of the pentacyclic oxindole alkaloids (POAs); mitraphylline and isomitraphylline in methanolic extracts by high-performance liquid chromatography (HPLC). Plantlets were cultivated on woody plant medium supplemented with 20 g.L -1 sucrose and 4.4 μM benzylaminopurine and incubated under a 16 h photoperiod for 45 days. SCoT analysis separated the genotypes into four divergent clusters and confirmed significant genetic diversity with up to 70% dissimilarity. Moreover, HPLC revealed considerable chemical variability and allowed the separation of the tested genotypes into high, medium and low producers of mitraphylline/isomitraphylline. Genotypes with the highest concentrations of POAs originated from the state of Acre and Amapá, while those with the lowest levels were from the state of Pará. The results demonstrate that the genetic diversity within the in vitro germplasm bank is sufficient to support breeding studies, selection of elite genotypes and the large-scale multiplication of plants that could serve as feedstock for the industrial-scale production of phytomedicines.
Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-03016-y.
(© King Abdulaziz City for Science and Technology 2021.)
Databáze: MEDLINE
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