Whole-Genome Sequencing of Gram-Negative Bacteria Isolated From Bovine Mastitis and Raw Milk: The First Emergence of Colistin mcr - 10 and Fosfomycin fosA5 Resistance Genes in Klebsiella pneumoniae in Middle East.

Autor:	Tartor YH; Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Abd El-Aziz NK; Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Gharieb RMA; Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., El Damaty HM; Animal Medicine Department (Infectious Diseases), Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Enany S; Microbiology and Immunology Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt., Soliman EA; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt., Abdellatif SS; Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Attia ASA; Veterinary Public Health Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Bahnass MM; Animal Medicine Department (Infectious Diseases), Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., El-Shazly YA; Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Elbediwi M; Animal Health Research Institute, Agriculture Research Center, Cairo, Egypt.; Institute of Veterinary Sciences, Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, China., Ramadan H; Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.; Bacterial Epidemiology and Antimicrobial Resistance Research Unit, US National Poultry Research Center, US Department of Agriculture, Agricultural Research Service (USDA-ARS), Athens, GA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Dec 08; Vol. 12, pp. 770813. Date of Electronic Publication: 2021 Dec 08 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.770813
Abstrakt:	Antimicrobial resistance is a major concern in the dairy industry. This study investigated the prevalence, antimicrobial resistance phenotypes, and genome sequencing of Gram-negative bacteria isolated from clinical ( n = 350) and subclinical ( n = 95) bovine mastitis, and raw unpasteurized milk ( n = 125). Klebsiella pneumoniae , Aeromonas hydrophila , Enterobacter cloacae (100% each), Escherichia coli (87.78%), and Proteus mirabilis (69.7%) were the most prevalent multidrug-resistant (MDR) species. Extensive drug-resistance (XDR) phenotype was found in P. mirabilis (30.30%) and E. coli (3.33%) isolates. Ten isolates (four E. coli , three Klebsiella species and three P. mirabilis ) that displayed the highest multiple antibiotic resistance (MAR) indices (0.54-0.83), were exposed to whole-genome sequencing (WGS). Two multilocus sequence types (MLST): ST2165 and ST7624 were identified among the sequenced E. coli isolates. Three E. coli isolates (two from clinical mastitis and one from raw milk) belonging to ST2165 showed similar profile of plasmid replicon types: IncFIA, IncFIB, IncFII, and IncQ1 with an exception to an isolate that contained IncR, whereas E. coli ST7624 showed a different plasmid profile including IncHI2, IncHI2A, IncI1α, and IncFII replicon types. ResFinder findings revealed the presence of plasmid-mediated colistin mcr - 10 and fosfomycin fosA 5 resistance genes in a K. pneumoniae (K1) isolate from bovine milk. Sequence analysis of the reconstructed mcr-10 plasmid from WGS of K1 isolate, showed that mcr-10 gene was bracketed by xer C and insertion sequence IS26 on an IncFIB plasmid. Phylogenetic analysis revealed that K1 isolate existed in a clade including mcr-10 -harboring isolates from human and environment with different STs and countries [United Kingdom (ST788), Australia (ST323), Malawi (ST2144), Myanmar (ST705), and Laos (ST2355)]. This study reports the first emergence of K. pneumoniae co-harboring mcr - 10 and fosA5 genes from bovine milk in the Middle East, which constitutes a public health threat and heralds the penetration of the last-resort antibiotics. Hence, prudent use of antibiotics in both humans and animals and antimicrobial surveillance plans are urgently required. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Tartor, Abd El-Aziz, Gharieb, El Damaty, Enany, Soliman, Abdellatif, Attia, Bahnass, El-Shazly, Elbediwi and Ramadan.)
Databáze:	MEDLINE
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