Indicators of Hypoxia Tolerance as Determined by Cellular Elements of Rat Blood.

Autor: Kondashevskaya MV; A.P. Avtsyn Research Institute of Human Morphology, Moscow, Russia., Artemieva KA; A.P. Avtsyn Research Institute of Human Morphology, Moscow, Russia., Aleksankina VV; A.P. Avtsyn Research Institute of Human Morphology, Moscow, Russia., Tikhonova NB; A.P. Avtsyn Research Institute of Human Morphology, Moscow, Russia., Boltovskaya MN; A.P. Avtsyn Research Institute of Human Morphology, Moscow, Russia.
Jazyk: angličtina
Zdroj: Journal of evolutionary biochemistry and physiology [J Evol Biochem Physiol] 2021; Vol. 57 (6), pp. 1231-1240. Date of Electronic Publication: 2021 Dec 20.
DOI: 10.1134/S002209302106003X
Abstrakt: Although hypoxia tolerance is mainly determined genetically, it is important to study individual variability of animal organisms in order to identify the factors that underlie their tolerance to hypoxic exposure. We investigated blood cell counts and coagulograms in Wistar rats as predictors allowing the animal population to be split into hypoxia-tolerant and hypoxia-intolerant individuals. The validity of the specific predictors' choice was proved by a coincidence between the population split in accordance with the detected individual parameters and the results of testing animals in a decompression chamber at a rarefaction corresponding to the "rise to an altitude" of 11500 m above sea level. Circulating blood cells were quantitatively assessed by eighteen indicators before and after hypoxic exposure. The differences between animals low-tolerant (LT), high-tolerant (HT), and medium-tolerant (MT) to hypoxia were determined by five indicators: white blood cell count (WBC), granulocyte count (Gran#), red blood cell count (RBC), reticulocyte count/percent (RTC), and mean corpuscular hemoglobin (MCH). The RBC, RTC, and MCH values in HT rats were significantly higher than in LT animals (by 1.4, 1.9, and 1.1 times, respectively). The WBC and Gran# values in HT rats were lower than in LT individuals. The hypoxia tolerance indices (HTI) were calculated using the original formula. It was established that in LT rats, the HTI ≤ 0.203, in HT rats ≥ 0.335, and in MT rats < 0.335 but > 0.203. After testing in a decompression chamber, the activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT) decreased, but the fibrinogen level increased. LT rats were characterized by the lowest APTT, TT, and PT values and the highest values of the fibrinogen level. Our results indicate that one of the most important mechanisms underlying a high hypoxia tolerance in rats consists in sustaining reciprocal relationships between the complex of RBC indicators, which tend to increase under hypoxia, and Gran# indicators, which tend to decrease after hypoxic exposure.
Competing Interests: CONFLICT OF INTERESTThe authors declare that they have neither evident nor potential conflict of interest related to the publication of this article.
(© Pleiades Publishing, Ltd. 2021.)
Databáze: MEDLINE
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