A role of Na+, K+ -ATPase in spatial memory deficits and inflammatory/oxidative stress after recurrent concussion in adolescent rats.

Autor: Cassol G; Exercise Biochemistry Laboratory, Center of Physical Education and Sports, Brazil; Postgraduate Program in Physical Education, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Cipolat RP; Exercise Biochemistry Laboratory, Center of Physical Education and Sports, Brazil; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Papalia WL; Exercise Biochemistry Laboratory, Center of Physical Education and Sports, Brazil; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Godinho DB; Exercise Biochemistry Laboratory, Center of Physical Education and Sports, Brazil; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Quines CB; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Nogueira CW; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Da Veiga M; Department of Morphology, Health Sciences Center, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Da Rocha MIUM; Department of Morphology, Health Sciences Center, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Furian AF; Laboratory of Neurotoxicity and Psychopharmacology, Health Sciences Center, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Oliveira MS; Laboratory of Neurotoxicity and Psychopharmacology, Health Sciences Center, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Fighera MR; Exercise Biochemistry Laboratory, Center of Physical Education and Sports, Brazil; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Department of Internal Medicine and Pediatrics, Health Sciences Center, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil., Royes LFF; Exercise Biochemistry Laboratory, Center of Physical Education and Sports, Brazil; Postgraduate Program in Physical Education, Brazil; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Brazil; Federal University of Santa Maria - UFSM, Santa Maria, RS, Brazil. Electronic address: nandoroyes@yahoo.com.br.
Jazyk: angličtina
Zdroj: Brain research bulletin [Brain Res Bull] 2022 Mar; Vol. 180, pp. 1-11. Date of Electronic Publication: 2021 Dec 23.
DOI: 10.1016/j.brainresbull.2021.12.009
Abstrakt: Sports-related concussions are particularly common during adolescence, and there is insufficient knowledge about how recurrent concussions in this phase of life alter the metabolism of essential structures for memory in adulthood. In this sense, our experimental data revealed that seven recurrent concussions (RC) in 35-day-old rats decreased short-term and long-term memory in the object recognition test (ORT) 30 days after injury. The RC protocol did not alter motor and anxious behavior and the immunoreactivity of brain-derived neurotrophic factor (BDNF) in the cerebral cortex. Recurrent concussions induced the inflammatory/oxidative stress characterized here by increased glial fibrillary acidic protein (GFAP), interleukin 1β (IL 1β), 4-hydroxynonenal (4 HNE), protein carbonyl immunoreactivity, and 2',7'-dichlorofluorescein diacetate oxidation (DCFH) levels and lower total antioxidant capacity (TAC). Inhibited Na + ,K + -ATPase activity (specifically isoform α 2/3 ) followed by K m (Michaelis-Menten constant) for increased ATP levels and decreased immunodetection of alpha subunit of this enzyme, suggesting that cognitive impairment after RC is caused by the inability of surviving neurons to maintain ionic gradients in selected targets to inflammatory/oxidative damage, such as Na,K-ATPase activity.
(Copyright © 2021. Published by Elsevier Inc.)
Databáze: MEDLINE
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