Microbiological and Clinical Effects of a Proanthocyanidin-enriched Extract from Rumex acetosa in Periodontally Healthy Carriers of Porphyromonas gingivalis: a Randomized Controlled Pilot Study.
| Autor: | Selbach S; Adelaide Dental School, The University of Adelaide, Adelaide, Australia., Klocke A; Private Practice, Krefeld, Germany., Peters U; Department of Periodontics, Preventive and Restorative Dentistry, University Medical Center Eppendorf, Hamburg, Germany., Beckert S; Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany., Watt RM; Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Peoples' Republic of China., Tong R; Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Peoples' Republic of China., Flemmig TF; Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Peoples' Republic of China., Hensel A; Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany., Beikler T; Department of Periodontics, Preventive and Restorative Dentistry, University Medical Center Eppendorf, Hamburg, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Planta medica [Planta Med] 2023 Sep; Vol. 89 (11), pp. 1052-1062. Date of Electronic Publication: 2021 Dec 25. |
| DOI: | 10.1055/a-1728-2249 |
| Abstrakt: | Rumex acetosa significantly inhibits the adhesion of Porphyromonas gingivalis ( P. g. ) to eukaryotic host cells in vitro . The objective of this randomized placebo-controlled pilot-trial was to analyze effects of a mouth rinse containing 0.8% (w/w) of a quantified proanthocyanidin-enriched extract from Rumex acetosa (RA1) on microbiological, clinical, and cytological parameters in systemically healthy individuals without history of periodontitis, harboring P. g. intraorally. 35 subjects received a supragingival debridement (SD) followed by mouth rinsing (3 times daily) with either RA1 mouth rinse solution (test) or placebo (control) for 7 days as adjunct to routine oral hygiene. Supragingival biofilm samples were taken at screening visit, baseline (BL), 2, 4, 7 and 14 days after SD. P. g. and 11 other oral microorganisms were detected and quantified by rtPCR. Changes in the oral microbiota composition of one test and one control subject were assessed via high throughput 16S rRNS gene amplicon sequencing. Approximal Plaque Index (API) and the modified Sulcular Bleeding Index (SBI) were assessed at BL, 7- and 14-days following SD. Brush biopsies were taken at BL and 14 d following SD. Intergroup comparisons revealed no significant microbiological, cytological, and clinical differences at any timepoint. However, a significant reduction in SBI at day 14 ( p = 0.003) and API at day 7 ( p = 0.02) and day 14 ( p = 0.009) was found in the test group by intragroup comparison. No severe adverse events were observed. The results indicate that RA1 mouth rinse is safe but does not seem to inhibit colonization of P. g. or improve periodontal health following SD. Competing Interests: The authors T. B. and A. H. filed a patent application “Use of proanthocyanidins for production of an antiadhesive preparation”, priority DE/14.09.09/DEA102009027696, on the 14.09.2009. However, the authors state that this application had no impact on the study protocol, its execution and evaluation and TB and AH refrained from data collection and data analysis. (Thieme. All rights reserved.) |
| Databáze: | MEDLINE |
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