A low-cost, portable, and practical LAMP device for point-of-diagnosis in the field.

Autor: Vural Kaymaz S; Mechatronics Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey., Ergenç AF; Department of Control and Automation Eng., Faculty of Electrical and Electronics Engineering, Istanbul Technical University, Istanbul, Turkey., Aytekin AÖ; Genetics and Bioengineering Program, Faculty of Engineering, Yeditepe University, Istanbul, Turkey., Lucas SJ; Molecular Biology and Genomics, Sabanci University Nanotechnology Research and Application Center, Istanbul, Turkey., Elitas M; Mechatronics Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey.; Molecular Biology and Genomics, Sabanci University Nanotechnology Research and Application Center, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2022 Mar; Vol. 119 (3), pp. 994-1003. Date of Electronic Publication: 2022 Jan 07.
DOI: 10.1002/bit.28025
Abstrakt: Transition of rapid, ready-to-use, and low-cost nucleic acid-based detection technologies from laboratories to points of sample collection has drastically accelerated. However, most of these approaches are still incapable of diagnosis starting from sampling through nucleic acid isolation and detection in the field. Here we developed a simple, portable, low-cost, colorimetric, and remotely controllable platform for reliable, high-throughput, and rapid diagnosis using loop-mediated isothermal amplification (LAMP) assays. It consists of a thermally isolated cup, low-cost electronic components, a polydimethylsiloxane sample well, and a fast prototyped case that covers electronic components. The steady-state temperature error of the system is <1%. We performed LAMP, Colony-LAMP, and Colony polymerase chain reactions (PCRs) using the yaiO 2 primer set for Escherichia coli and Pseudomonas aeruginosa samples at 65°C and 30 min. We detected the end-point colorimetric readouts by the naked eye under day light. We confirmed the specificity and sensitivity of our approach using pure genomic DNA and crude bacterial colonies. We benchmarked our Colony-LAMP detection against Colony PCR. The number of samples tested can easily be modified for higher throughput in our system. We strongly believe that our platform can greatly contribute rapid and reliable diagnosis in versatile operational environments.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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