Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system.

Autor:	Vimonish R; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA., Dinkel KD; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA., Fry LM; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA.; USDA-ARS-Animal Diseases Research Unit, Pullman, WA, 99164-6630, USA., Johnson WC; USDA-ARS-Animal Diseases Research Unit, Pullman, WA, 99164-6630, USA., Capelli-Peixoto J; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA., Bastos RG; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA., Scoles GA; USDA-ARS-Animal Diseases Research Unit, Pullman, WA, 99164-6630, USA.; Invasive Insect Biocontrol and Behavior Lab, USDA, ARS, Beltsville, MD, 20705, USA., Knowles DP; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA., Madder M; Clinglobal, Black River, Tamarin, 90903, Mauritius., Chaka G; Centre for Ticks and Tick-Borne Diseases, 309200, Lilongwe, Malawi., Ueti MW; Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA. massaro.ueti@usda.gov.; USDA-ARS-Animal Diseases Research Unit, Pullman, WA, 99164-6630, USA. massaro.ueti@usda.gov.; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, 99164, USA. massaro.ueti@usda.gov.
Jazyk:	angličtina
Zdroj:	Parasites & vectors [Parasit Vectors] 2021 Dec 24; Vol. 14 (1), pp. 616. Date of Electronic Publication: 2021 Dec 24.
DOI:	10.1186/s13071-021-05120-7
Abstrakt:	Background: Vector-borne diseases pose an increasing threat to global food security. Vaccines, diagnostic tests, and therapeutics are urgently needed for tick-borne diseases that affect livestock. However, the inability to obtain significant quantities of pathogen stages derived from ticks has hindered research. In vitro methods to isolate pathogens from infected tick vectors are paramount to advance transcriptomic, proteomic, and biochemical characterizations of tick-borne pathogens. Methods: Nymphs of Rhipicephalus appendiculatus were infected with Theileria parva by feeding on a calf during an acute infection. Isolation of sporozoites was accomplished by feeding infected adult ticks on an in vitro tick feeding system. Sporozoite viability was tested using in vitro bovine lymphocytes. Results: We isolated infectious T. parva sporozoites secreted into an in vitro tick feeding system. Infected adult R. appendiculatus ticks attached to and successfully fed on silicone membranes in the in vitro tick feeding system. Bovine blood in the receptacle was replaced with cell-free medium and the ticks were allowed to feed for 3 h to collect secreted T. parva sporozoites. Secreted sporozoites infected in vitro bovine lymphocytes, demonstrating that isolated sporozoites remained viable and infectious. Conclusions: This work is the first to report the isolation of mature infectious T. parva sporozoites using an in vitro tick feeding system, which represents a significant step towards the development of a more efficient control strategy for T. parva. Isolation of infectious tick-stage parasites will facilitate the examination of the vector-pathogen interface, thereby accelerating the development of next-generation vaccines and treatment interventions for tick-borne pathogens. (© 2021. The Author(s).)
Databáze:	MEDLINE
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