Evaluation of the biological and physicochemical properties of calcium silicate-based and epoxy resin-based root canal sealers.

Autor: Ferreira GC; Post Graduate Program in Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., Pinheiro LS; Post Graduate Program in Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., Nunes JS; Post Graduate Program in Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., de Almeida Mendes R; Post Graduate Program in Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., Schuster CD; University of Rio Grande do Sul, Porto Alegre, Brazil., Soares RG; College of Dentistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada., Kopper PMP; Department of Conservative Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., de Figueiredo JAP; Department of Morphology - ICBS, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., Grecca FS; Department of Conservative Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2022 Jun; Vol. 110 (6), pp. 1344-1353. Date of Electronic Publication: 2021 Dec 24.
DOI: 10.1002/jbm.b.35004
Abstrakt: Biocompatibility, dimensional stability, radiopacity, flow, and low solubility are the characteristics of an ideal endodontic sealer. This study evaluated and compared in vivo and in vitro biological and physicochemical properties of calcium silicate-based sealers: Sealer Plus BC (BC), MTA Fillapex (MF); and resin-based sealers: AH Plus (AHP) and Sealer Plus (SP). Apical papilla cells were exposed to sealer extracts and subjected to MTT, SRB, scratch, alkaline phosphatase enzyme activity (ALP) and Alizarin red staining (ALZ) assays. Sealers were histologically evaluated in connective tissue of Wistar rats in different periods. Radiopacity, film thickness, flow, setting time, pH and element analyses were investigated. BC had better results compared to AHP and MF at hour 72 for MTT assay (p < .05), and the highest cell viability under SRB (p < .05). All sealers presented ALP activity. BC presented the highest mineralized deposition under ALZ (p < .05). BC and MF promoted wound healing. All sealers induced an initial inflammation reaction that decreased over time. Eosinophils were observed at day 7 in MF (p < .05). Despite MF did not present final setting time, the sealers properties were in accordance to ISO 6876/2012 and ASTM C266-08. All sealers presented cell viability and biocompatibility. BC presented higher pH values and bioactivity. The materials tested showed physico-chemical properties in accordance with standards, except for MF setting time.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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