Evaluating the presence of Mycoplasma hyorhinis, Fusobacterium nucleatum, and Helicobacter pylori in biopsies of patients with gastric cancer.

Autor: Nascimento Araujo CD; Department of Microbiology, Institute of Biomedical Sciences, ICB/USP, University of São Paulo, São Paulo, Brazil., Amorim AT; Department of Microbiology, Institute of Biomedical Sciences, ICB/USP, University of São Paulo, São Paulo, Brazil., Barbosa MS; Department of Microbiology, Institute of Biomedical Sciences, ICB/USP, University of São Paulo, São Paulo, Brazil., Alexandre JCPL; Department of Animal Health, Faculty of Veterinary Medicine, José Eduardo dos Santos University, Huambo, Angola., Campos GB; Department of Microbiology, Institute of Biomedical Sciences, ICB/USP, University of São Paulo, São Paulo, Brazil.; Multidisciplinary Health Institute /Campus Anísio Teixeira, IMS/CAT - UFBA, Federal University of Bahia, Vitória da Conquista, Brazil., Macedo CL; Micro - Pathological Anatomy and Cytopathology Service, Vitória da Conquista, Brazil., Marques LM; Department of Microbiology, Institute of Biomedical Sciences, ICB/USP, University of São Paulo, São Paulo, Brazil. lmirandamarques@gmail.com.; Multidisciplinary Health Institute /Campus Anísio Teixeira, IMS/CAT - UFBA, Federal University of Bahia, Vitória da Conquista, Brazil. lmirandamarques@gmail.com., Timenetsky J; Department of Microbiology, Institute of Biomedical Sciences, ICB/USP, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Infectious agents and cancer [Infect Agent Cancer] 2021 Dec 23; Vol. 16 (1), pp. 70. Date of Electronic Publication: 2021 Dec 23.
DOI: 10.1186/s13027-021-00410-2
Abstrakt: Background: Gastric cancer is the third leading cause of cancer-related deaths worldwide and has been associated with infections that may promote tumour progression. Accordingly, we analysed the presence of Mollicutes, Mycoplasma hyorhinis, Fusobacterium nucleatum and Helicobacter pylori in gastric cancer tissues and evaluated their correlation with clinicopathological factors.
Methods: Using a commercial kit, DNA were extracted from 120 gastric samples embedded in paraffin: 80 from patients with gastric cancer and 40 from cancer free patients, dating from 2006 to 2016. Mollicutes and H. pylori were detected by PCR; F. nucleatum and M. hyorhinis were detected by qPCR, together with immunohistochemistry for the latter bacteria.
Results: Mollicutes were detected in the case and control groups (12% and 2.5%) and correlated with the papillary histologic pattern (P = 0.003), likely due to cell transformation promoted by Mollicutes. M. hyorhinis was detected in the case and control group but was not considered a cancer risk factor. H. pylori was detected at higher loads in the case compared to the control group (8% and 22%, P = 0.008) and correlated with metastasis (P = 0.024), lymphatic invasion (P = 0.033), tumour of diffused type (P = 0.028), and histopathological grading G1/G2 (P = 0.008). F. nucleatum was the most abundant bacteria in the case group, but was also detected in the control group (26% and 2.5%). It increased the cancer risk factor (P = 0.045, OR = 10.562, CI95% = 1.057-105.521), and correlated with old age (P = 0.030) and tumour size (P = 0.053). Bacterial abundance was significantly different between groups (P = 0.001).
Conclusion: Our findings could improve the control and promote our understanding of opportunistic bacteria and their relevance to malignant phenotypes.
(© 2021. The Author(s).)
Databáze: MEDLINE
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