Delineation of the Ancestral Tus-Dependent Replication Fork Trap.

Autor: Toft CJ; Molecular and Cell Biology, College of Public Health, Medical and Veterinary Sciences, James Cook University, Douglas, QLD 4811, Australia.; Centre of Tropical Bioinformatics and Molecular Biology, James Cook University, Douglas, QLD 4811, Australia., Moreau MJJ; Molecular and Cell Biology, College of Public Health, Medical and Veterinary Sciences, James Cook University, Douglas, QLD 4811, Australia., Perutka J; Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712, USA., Mandapati S; Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712, USA., Enyeart P; Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712, USA., Sorenson AE; Molecular and Cell Biology, College of Public Health, Medical and Veterinary Sciences, James Cook University, Douglas, QLD 4811, Australia., Ellington AD; Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712, USA., Schaeffer PM; Molecular and Cell Biology, College of Public Health, Medical and Veterinary Sciences, James Cook University, Douglas, QLD 4811, Australia.; Centre of Tropical Bioinformatics and Molecular Biology, James Cook University, Douglas, QLD 4811, Australia.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 16; Vol. 22 (24). Date of Electronic Publication: 2021 Dec 16.
DOI: 10.3390/ijms222413533
Abstrakt: In Escherichia coli , DNA replication termination is orchestrated by two clusters of Ter sites forming a DNA replication fork trap when bound by Tus proteins. The formation of a 'locked' Tus- Ter complex is essential for halting incoming DNA replication forks. However, the absence of replication fork arrest at some Ter sites raised questions about their significance. In this study, we examined the genome-wide distribution of Tus and found that only the six innermost Ter sites ( TerA-E and G ) were significantly bound by Tus. We also found that a single ectopic insertion of TerB in its non-permissive orientation could not be achieved, advocating against a need for 'back-up' Ter sites. Finally, examination of the genomes of a variety of Enterobacterales revealed a new replication fork trap architecture mostly found outside the Enterobacteriaceae family. Taken together, our data enabled the delineation of a narrow ancestral Tus-dependent DNA replication fork trap consisting of only two Ter sites.
Databáze: MEDLINE
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