Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus.

Autor: Toshchakov SV; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia., Izotova AO; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia., Vinogradova EN; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia.; Faculty of Biology, Lomonosov Moscow State University, 1-12 Leninskie Gory, Moscow 119991, Russia., Kachmazov GS; Faculty of Chemistry, Biology and Biotechnology, North Ossetian State University Named after K.L. Khetagurov, Vatutina str., 44-46, Vladikavkaz 362025, Russia., Tuaeva AY; National Research Center Kurchatov Institute-GOSNIIGENETIKA, 1st Dorozhny Pr., 1, Moscow 117545, Russia., Abaev VT; Faculty of Chemistry, Biology and Biotechnology, North Ossetian State University Named after K.L. Khetagurov, Vatutina str., 44-46, Vladikavkaz 362025, Russia., Evteeva MA; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia., Gunitseva NM; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia., Korzhenkov AA; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia., Elcheninov AG; Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS, 60-let Oktyzbrya Av., 7/2, Moscow 119071, Russia., Patrushev MV; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Ac. Kurchatov Square, 1, Moscow 123098, Russia., Kublanov IV; Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS, 60-let Oktyzbrya Av., 7/2, Moscow 119071, Russia.
Jazyk: angličtina
Zdroj: Biology [Biology (Basel)] 2021 Dec 20; Vol. 10 (12). Date of Electronic Publication: 2021 Dec 20.
DOI: 10.3390/biology10121352
Abstrakt: The Greater Caucasus is a part of seismically active Alpine-Himalayan orogenic belt and has been a center of significant volcanic activity during the Quaternary period. That led to the formation of the number of hydrothermal habitats, including subterranean thermal aquifers and surface hot springs. However, there are only a limited number of scientific works reporting on the microbial communities of these habitats. Moreover, all these reports concern only studies of specific microbial taxa, carried out using classical cultivation approaches. In this work, we present first culture-independent study of hydrotherms in the Republic of North Ossetia-Alania, located in the southern part of the North Caucasus. Using 16S metabarcoding, we analyzed the composition of the microbial communities of two subterranean thermal aquifers and terrestrial hot springs of the Karmadon valley. Analysis of correlations between the chemical composition of water and the representation of key taxa allowed us to identify the key factors determining the formation of microbial communities. In addition, we were able to identify a significant number of highly abundant deep phylogenetic lineages. Our study represents a first glance on the thermophilic microbial communities of the North Caucasus and may serve as a basis for further microbiological studies of the extreme habitats of this region.
Databáze: MEDLINE
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