Metabolic Fluctuations in the Human Stool Obtained from Blastocystis Carriers and Non-Carriers.

Autor:	Betts EL; Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, Kent, UK., Newton JM; Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, Kent, UK., Thompson GS; Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, Kent, UK.; Wellcome Trust Biomolecular NMR Facility, School of Biosciences, University of Kent, Canterbury CT2 7NJ, Kent, UK., Sarzhanov F; Faculty of Medicine, Akhmet Yassawi International Kazakh-Turkish University, Turkestan 161200, Kazakhstan., Jinatham V; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand., Kim MJ; Department of Parasitology and Tropical Medicine, Chonnam National University Medical School, Hwasun-gun 58128, Jeollanam-do, Korea., Popluechai S; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.; Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai 57100, Thailand., Dogruman-Al F; Division of Medical Parasitology, Department of Medical Microbiology, School of Medicine, Gazi University, Ankara 06490, Turkey., Won EJ; Department of Parasitology and Tropical Medicine, Chonnam National University Medical School, Hwasun-gun 58128, Jeollanam-do, Korea.; Department of Laboratory Medicine, Chonnam National University Hwasun Hospital, Hwasun-gun, Gwangju 58128, Jeollanam-do, Korea., Gentekaki E; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.; Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai 57100, Thailand., Tsaousis AD; Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, Kent, UK.
Jazyk:	angličtina
Zdroj:	Metabolites [Metabolites] 2021 Dec 17; Vol. 11 (12). Date of Electronic Publication: 2021 Dec 17.
DOI:	10.3390/metabo11120883
Abstrakt:	Blastocystis is an obligate anaerobic microbial eukaryote that frequently inhabits the gastrointestinal tract. Despite this prevalence, very little is known about the extent of its genetic diversity, pathogenicity, and interaction with the rest of the microbiome and its host. Although the organism is morphologically static, it has no less than 28 genetically distinct subtypes (STs). Reports on the pathogenicity of Blastocystis are conflicting. The association between Blastocystis and intestinal bacterial communities is being increasingly explored. Nonetheless, similar investigations extending to the metabolome are non-existent.Using established NMR metabolomics protocols in 149 faecal samples from individuals from South Korea ( n = 38), Thailand ( n = 44) and Turkey ( n = 69), we have provided a snapshot of the core metabolic compounds present in human stools with ( B+ ) and without ( B- ) Blastocystis . Samples included hosts with gastrointestinal symptoms and asymptomatics. A total of nine, 62 and 98 significant metabolites were associated with Blastocystis carriage in the South Korean, Thai and Turkish sample sets respectively, with a number of metabolites increased in colonised groups. The metabolic profiles of B+ and B- samples from all countries were distinct and grouped separately in the partial least squares-discriminant analysis (PLS-DA). Typical inflammation-related metabolites negatively associated with Blastocystis positive samples. This data will assist in directing future studies underlying the involvement of Blastocystis in physiological processes of both the gut microbiome and the host. Future studies using metabolome and microbiome data along with host physiology and immune responses information will contribute significantly towards elucidating the role of Blastocystis in health and disease.
Databáze:	MEDLINE
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