| Autor: |
Mattison CP; Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture, New Orleans, LA 70124, USA., Vant-Hull B; Contiguity Consulting, Boulder, CO 80302, USA., de Castro ACR; Embrapa Agroindústria Tropical, Fortaleza 60511-110, CE, Brazil., Chial HJ; BioMed Bridge LLC., 3700 Quebec Street, Suite 100-230, Denver, CO 80207, USA., Bren-Mattison Y; Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.; New Orleans Louisiana Neuroendocrine Tumor Specialists (NOLANETS), Ochsner Medical Center, Kenner, LA 70065, USA., Bechtel PJ; Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture, New Orleans, LA 70124, USA., de Brito ES; Embrapa Agroindústria Tropical, Fortaleza 60511-110, CE, Brazil. |
| Abstrakt: |
Ana o 3 is an immuno-dominant cashew nut allergen. Four monoclonal antibodies to Ana o 3 (2H5, 6B9C1, 19C9A2, and 5B7F8) were characterized by ELISA and in silico modeling. The 2H5 antibody was the only antibody specific for cashew nut extract. In addition to cashew nut extract, the 6B9C1 and 19C9A2 antibodies recognized pistachio extract, and the 5B7F8 recognized pecan extract. All four antibodies recognized both recombinant Ana o 3.0101 and native Ana o 3. ELISA assays following treatment of purified Ana o 3 with a reducing agent indicated that the 6B9C1 and 19C9A2 antibodies likely recognize conformational epitopes, while the 2H5 and 5B7F8 antibodies likely recognize linear epitopes. In silico modeling predicted distinct epitopes for each of the anti-Ana o 3 antibodies. Screening extracts from 11 Brazilian cashew nut cultivars using all four antibodies showed slight differences in Ana o 3 bindings, demonstrating that these antibodies could identify cultivars with varying allergen content. |
