Synergistic stimulation of surface topography and biphasic electric current promotes muscle regeneration.

Autor:	Jun I; Environmental Safety Group, Korea Institute of Science & Technology Europe (KIST-EUROPE), Saarbrücken, 66123, Germany., Li N; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, 02841, Republic of Korea., Shin J; Department of Medical Sciences, Graduate School of Medicine, Korea University, Seoul, 02841, Republic of Korea., Park J; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology (KIST), Seoul, 02792, Republic of Korea., Kim YJ; Environmental Safety Group, Korea Institute of Science & Technology Europe (KIST-EUROPE), Saarbrücken, 66123, Germany., Jeon H; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology (KIST), Seoul, 02792, Republic of Korea., Choi H; Department of Medical Sciences, Graduate School of Medicine, Korea University, Seoul, 02841, Republic of Korea., Cho JG; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, 02841, Republic of Korea., Chan Choi B; Laser Surface Texturing Group, AYECLUS, Gyeonggi-do, 14255, Republic of Korea., Han HS; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology (KIST), Seoul, 02792, Republic of Korea., Song JJ; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, 02841, Republic of Korea.
Jazyk:	angličtina
Zdroj:	Bioactive materials [Bioact Mater] 2021 Oct 19; Vol. 11, pp. 118-129. Date of Electronic Publication: 2021 Oct 19 (Print Publication: 2022).
DOI:	10.1016/j.bioactmat.2021.10.015
Abstrakt:	Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment. This study focuses on the application of topographical and electrical field stimuli to human myogenic precursor cell (hMPC) cultures to assess the influences of the adherent direction, proliferation, and differentiation, and induce preconditioning-induced therapeutic benefits. First, a topographical surface of commercially available culture dishes was achieved by femtosecond laser texturing. The detachable biphasic electrical current system was then applied to the hMPCs cultured on laser-textured culture dishes. Laser-textured topographies were remarkably effective in inducing the assembly of hMPC myotubes by enhancing the orientation of adherent hMPCs compared with flat surfaces. Furthermore, electrical field stimulation through laser-textured topographies was found to promote the expression of myogenic regulatory factors compared with nonstimulated cells. As such, we successfully demonstrated that the combined stimulation of topographical and electrical cues could effectively enhance the myogenic maturation of hMPCs in a surface spatial and electrical field-dependent manner, thus providing the basis for therapeutic strategies. Competing Interests: The authors declare no conflict of interest. (© 2021 The Authors.)
Databáze:	MEDLINE
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