Partial characterization of viscous exopolymer produced by Alkalihalobacillus sp. strain SB-D (KJ372395) with emulsification and adhesive properties.

Autor: Borkar SR; Department of Microbiology, Goa University, Taleigao Plateau, Goa, India. sunib567@gmail.com.; UG and PG Department of Microbiology, P.E.S's R.S.N College of Arts and Science, Farmagudi, Ponda, Goa, India. sunib567@gmail.com., Bhosle SN; Department of Microbiology, Goa University, Taleigao Plateau, Goa, India.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2021 Dec 21; Vol. 204 (1), pp. 48. Date of Electronic Publication: 2021 Dec 21.
DOI: 10.1007/s00203-021-02679-8
Abstrakt: Twenty-two alkaliphilic bacteria were isolated from spent waters of an agrochemical factory, Goa, India, and screened for exopolymer (EP) production on Horikoshi I/II media. Strain SB-D produced highly viscous cell-bound and extracellular EP quantified by alcian blue adsorption assay. The 16S rRNA gene sequence of strain SB-D showed 97.34% similarity to Alkalihalobacillus lehensis strain MLB T . The strain maximally produced EP (10.3 g/L) at pH 10.3, 25 °C after 16 h growth on shaker, followed by 4 h at static condition with 4% inoculum in the presence of 3% glucose and 0.1% yeast extract. The EP precipitated using isopropanol contained carbohydrates (212 µg/mg), lipids (130 µg/mg), and proteins (86.16 µg/mg) as major components. Further, gas chromatography detected the presence of D-glucose, D-arabinose, D-mannose, D-galactose, D-xylose and D-ribose. Preliminary studies on EP characterization revealed emulsification and adhesive properties of the exopolymer.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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