Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition.

Autor:	Dimitriu T; ESI, Biosciences, University of Exeter, TR10 9FE Penryn, UK. Electronic address: t.dimitriu@exeter.ac.uk., Kurilovich E; Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia., Łapińska U; Living Systems Institute and Biosciences, University of Exeter, EX4 4QD Exeter, UK., Severinov K; Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia; Waksman Institute of Microbiology, Piscataway, NJ 08854, USA; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 119334, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia., Pagliara S; Living Systems Institute and Biosciences, University of Exeter, EX4 4QD Exeter, UK., Szczelkun MD; DNA-Protein Interactions Unit, School of Biochemistry, University of Bristol, BS8 1TD Bristol, UK., Westra ER; ESI, Biosciences, University of Exeter, TR10 9FE Penryn, UK. Electronic address: e.r.westra@exeter.ac.uk.
Jazyk:	angličtina
Zdroj:	Cell host & microbe [Cell Host Microbe] 2022 Jan 12; Vol. 30 (1), pp. 31-40.e5. Date of Electronic Publication: 2021 Dec 20.
DOI:	10.1016/j.chom.2021.11.014
Abstrakt:	Phages impose strong selection on bacteria to evolve resistance against viral predation. Bacteria can rapidly evolve phage resistance via receptor mutation or using their CRISPR-Cas adaptive immune systems. Acquisition of CRISPR immunity relies on the insertion of a phage-derived sequence into CRISPR arrays in the bacterial genome. Using Pseudomonas aeruginosa and its phage DMS3vir as a model, we demonstrate that conditions that reduce bacterial growth rates, such as exposure to bacteriostatic antibiotics (which inhibit cell growth without killing), promote the evolution of CRISPR immunity. We demonstrate that this is due to slower phage development under these conditions, which provides more time for cells to acquire phage-derived sequences and mount an immune response. Our data reveal that the speed of phage development is a key determinant of the evolution of CRISPR immunity and suggest that use of bacteriostatic antibiotics can trigger elevated levels of CRISPR immunity in human-associated and natural environments. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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