Helicid Reverses the Effect of Overexpressing NCALD , Which Blocks the sGC/cGMP/PKG Signaling Pathway in the CUMS-Induced Rat Model.

Autor: Zhang XT; The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, Anhui, China.; Wannan Medical College, Wuhu 241002, Anhui, China., Zhang Y; The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, Anhui, China.; Wannan Medical College, Wuhu 241002, Anhui, China., Zhang YX; Wannan Medical College, Wuhu 241002, Anhui, China., Jiang ZY; The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, Anhui, China.; Wannan Medical College, Wuhu 241002, Anhui, China., Yang H; Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Wannan Medical College), Wuhu, China.; Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China., Jiang L; Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Wannan Medical College), Wuhu, China.; Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China., Yang B; Department of Clinical Pharmacy, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, Anhui, China., Tong JC; The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, Anhui, China.; Wannan Medical College, Wuhu 241002, Anhui, China.
Jazyk: angličtina
Zdroj: Journal of healthcare engineering [J Healthc Eng] 2021 Dec 11; Vol. 2021, pp. 7168397. Date of Electronic Publication: 2021 Dec 11 (Print Publication: 2021).
DOI: 10.1155/2021/7168397
Abstrakt: Background: Increasing evidence has shown that apoptosis in the hippocampus is closely related to depressive-like behavior. We previously reported that helicid had good antidepressant activities, which manifested as the alleviation of depression-like behaviors and the reversal of the high expression of neurocalcin delta ( NCALD ) in chronic unpredictable mild stress (CUMS) rats. The aim of this study was, therefore, to characterize the antidepressant-like effects and underlying mechanism of helicid on CUMS rats by silencing NCALD and using rescue experiments.
Methods: We developed the CUMS rat model using CUMS stimulation from week 0 to week 6. The rats were treated with helicid, or NCALD silenced, then we overexpressed NCALD using adeno-associated virus. We also measured the protein levels of sGC α 1, sGC β 1, PKG1/2, and cleaved caspase-3 in hippocampal tissues using western blotting and measured cGMP using an ELISA.
Results: Treating CUMS rats by silencing NCALD or by the administration of helicid improved the depressive-like behavior. The levels of proteins, including sGC, PKG, cleaved caspase-3, and cGMP, in hippocampus all decreased. NCALD overexpression reversed these decreases and reversed the alleviation of depression-like behaviors in CUMS rats. Limitation . We only detected the antidepressant effects of helicid in the hippocampus; therefore, other parts of brain should also be studied.
Conclusions: Inhibition of NCALD , as well as helicid administration, alleviated antidepressant-like behavior by regulating the expressions of apoptotic cytokines and the sGC/cGMP/PKG signaling pathway. Overexpressing NCALD reversed the amelioration effects of silenced NCALD and helicid administration.
Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper.
(Copyright © 2021 Xiao-Tong Zhang et al.)
Databáze: MEDLINE
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