Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366.

Autor: Liu YJ; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China., Li D; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China., Gong J; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China., Wang YB; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China., Chen ZB; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China., Pang BS; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China., Chen XC; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China., Gao JG; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China., Yang WB; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China., Zhang FT; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. lyezh@163.com., Tang YM; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. tangyimiao@126.com.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China. tangyimiao@126.com., Zhao CP; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. cp_zhao@vip.sohu.com.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China. cp_zhao@vip.sohu.com., Gao SQ; Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. gshiq@126.com.; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China. gshiq@126.com.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2021 Dec 20; Vol. 22 (1), pp. 911. Date of Electronic Publication: 2021 Dec 20.
DOI: 10.1186/s12864-021-08163-3
Abstrakt: Background: Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 °C with 12 h of daylight) but fertile under normal temperature (20 °C with 12 h of daylight).
Results: During meiosis, BS366 was defective in forming tetrads and dyads due to the abnormal cell plate. During pollen development, unusual vacuolated pollen that could not accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process, such as sister chromatid segregation and microtubule-based movement, were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold conditions. MethylRAD was used for reduced DNA methylation sequencing of BS366 spikes under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Differentially expressed and methylated genes were mainly involved in cell division.
Conclusions: These results indicated that the methylation of genes involved in carbon metabolism or fatty acid metabolism might contribute to male sterility in BS366 spikes, providing novel insight into the molecular mechanism of wheat male sterility.
(© 2021. The Author(s).)
Databáze: MEDLINE
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