Citrobacter rodentium(ϕStx2dact), a murine infection model for enterohemorrhagic Escherichia coli.

Autor: Thorpe CM; Tufts University School of Medicine, Tufts Medical Center, 800 Washington Street, Box 041, Boston, MA 02111, USA; Stuart B. Levy Center for Integrated Management of Antimicrobial Resistance, Tufts University, Boston, MA, USA. Electronic address: cthorpe@tuftsmedicalcenter.org., Pulsifer AR; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA., Osburne MS; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA., Vanaja SK; Department of Immunology, UConn Health School of Medicine, 263 Farmington Ave., Farmington, CT 06030, USA., Leong JM; Stuart B. Levy Center for Integrated Management of Antimicrobial Resistance, Tufts University, Boston, MA, USA; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA. Electronic address: john.leong@tufts.edu.
Jazyk: angličtina
Zdroj: Current opinion in microbiology [Curr Opin Microbiol] 2022 Feb; Vol. 65, pp. 183-190. Date of Electronic Publication: 2021 Dec 17.
DOI: 10.1016/j.mib.2021.11.013
Abstrakt: The formation of attaching and effacing (A/E) lesions on intestinal epithelium, combined with Shiga toxin production, are hallmarks of enterohemorrhagic Escherichia coli (EHEC) infection that can lead to lethal hemolytic uremic syndrome. Although an animal infection model that fully recapitulates human disease remains elusive, mice orally infected with Citrobacter rodentium(ϕStx2dact), a natural murine pathogen lysogenized with an EHEC-derived Shiga toxin 2-producing bacteriophage, develop intestinal A/E lesions and toxin-dependent systemic disease. This model has facilitated investigation of how: (A) phage gene expression and prophage induction contribute to disease and are potentially triggered by antibiotic treatment; (B) virulence gene expression is altered by microbiota and the colonic metabolomic milieu; and (C) innate immune signaling is affected by Stx. Thus, the model provides a unique tool for accessing diverse aspects of EHEC pathogenesis.
(Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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