RpoN/Sfa2-dependent activation of the Pseudomonas aeruginosa H2-T6SS and its cognate arsenal of antibacterial toxins.

Autor:	Allsopp LP; Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK.; National Heart and Lung Institute, Imperial College London, London, UK., Collins ACZ; National Heart and Lung Institute, Imperial College London, London, UK., Hawkins E; Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK., Wood TE; Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK., Filloux A; Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2022 Jan 11; Vol. 50 (1), pp. 227-243.
DOI:	10.1093/nar/gkab1254
Abstrakt:	Pseudomonas aeruginosa uses three type six secretion systems (H1-, H2- and H3-T6SS) to manipulate its environment, subvert host cells and for microbial competition. These T6SS machines are loaded with a variety of effectors/toxins, many being associated with a specific VgrG. How P. aeruginosa transcriptionally coordinates the main T6SS clusters and the multiple vgrG islands spread through the genome is unknown. Here we show an unprecedented level of control with RsmA repressing most known T6SS-related genes. Moreover, each of the H2- and H3-T6SS clusters encodes a sigma factor activator (SFA) protein called, Sfa2 and Sfa3, respectively. SFA proteins are enhancer binding proteins necessary for the sigma factor RpoN. Using a combination of RNA-seq, ChIP-seq and molecular biology approaches, we demonstrate that RpoN coordinates the T6SSs of P. aeruginosa by activating the H2-T6SS but repressing the H1- and H3-T6SS. Furthermore, RpoN and Sfa2 control the expression of the H2-T6SS-linked VgrGs and their effector arsenal to enable very effective interbacterial killing. Sfa2 is specific as Sfa3 from the H3-T6SS cannot complement loss of Sfa2. Our study further delineates the regulatory mechanisms that modulate the deployment of an arsenal of T6SS effectors likely enabling P. aeruginosa to adapt to a range of environmental conditions. (© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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