Autor: |
Pegorin Brasil GS; Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.; Department of Biotechnology and Bioprocess Engineering, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil., de Barros PP; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil.; Multicampi School of Medical Sciences, Federal University of Rio Grande do Norte (UFRN), Caico, Rio Grande do Norte, Brazil., Miranda MCR; Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Aveiro, Portugal., de Barros NR; Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, California, USA., Junqueira JC; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil., Gomez A; Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, California, USA., Herculano RD; Department of Biotechnology and Bioprocess Engineering, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil., de Mendonça RJ; Department of Biochemistry, Pharmacology and Physiology, Federal University of Triangulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil. |
Abstrakt: |
Natural latex serum (NLS) is one of the natural rubber latex fractions from Hevea brasiliensis tree, which is formed by centrifuged serum and is composed of proteins, acids, nucleotides, salts and carbohydrates. The proteins present in NLS have demonstrated several interesting biological properties, including angiogenic, healing, osteogenic, anti-inflammatory, antimicrobial, in addition to inducing neovascularization, bone formation and osseointegration. Thus, we proposed to characterize NLS by physicochemical techniques and to investigate the biocompatibility by toxicological assays and safety test in Galleria mellonella . Infrared spectrum showed vibrational bands characteristic of amide I, II and III that are linked to the protein content, which was confirmed by the High Performance Liquid Chromatography profile and by the Electrophoresis analysis. This material did not exhibit hemolytic (rate <0.5%) and cytotoxic effects (viability >70%) and was able to enhance the proliferation of fibroblasts (>600%) after 3 days. The pronounced proliferative effect observed in fibroblast cells can be explained by the presence of the fibroblast growth factor (FGF) like protein revealed by the Western blot test. Moreover, NLS did not provoke toxic effects (survival ∼ 80%) on the G. mellonella model, indicating that it is a biocompatible and safe material. |