Isolation and agricultural potential of penicillic acid against citrus canker.

Autor: Vieira G; Institute of Biosciences, Department of General and Applied Biology, São Paulo State University (UNESP), Rio Claro, Brazil., Khalil ZG; Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia., Capon RJ; Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia., Sette LD; Institute of Biosciences, Department of General and Applied Biology, São Paulo State University (UNESP), Rio Claro, Brazil., Ferreira H; Institute of Biosciences, Department of General and Applied Biology, São Paulo State University (UNESP), Rio Claro, Brazil., Sass DC; Institute of Biosciences, Department of General and Applied Biology, São Paulo State University (UNESP), Rio Claro, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2022 Apr; Vol. 132 (4), pp. 3081-3088. Date of Electronic Publication: 2022 Jan 05.
DOI: 10.1111/jam.15413
Abstrakt: Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment.
Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml - 1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm 2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis.
Conclusion: Penicillic acid can be an alternative against citrus canker.
Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.
(© 2021 The Society for Applied Microbiology.)
Databáze: MEDLINE
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