Photo-Cross-Linkable Human Albumin Colloidal Gels Facilitate In Vivo Vascular Integration for Regenerative Medicine.

Autor:	Yoon H; College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Seoul 05029, Republic of Korea., Lee H; College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Seoul 05029, Republic of Korea., Shin SY; Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Seoul 05029, Republic of Korea., Jodat YA; Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, Massachusetts 02139, United States., Jhun H; Technical Assistance Center, Korea Food Research Institute, Jeonbuk 55365, Republic of Korea., Lim W; Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Seoul 05029, Republic of Korea., Seo JW; Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Seoul 05029, Republic of Korea., Kim G; Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Seoul 05029, Republic of Korea., Mun JY; Neural Circuit Research Group, Korea Brain Research Institute (KBRI), Daegu 41068, Republic of Korea., Zhang K; Department of Mechanical and Industrial Engineering, Northeastern University, Boston, Massachusetts 02115, United States., Wan KT; Department of Mechanical and Industrial Engineering, Northeastern University, Boston, Massachusetts 02115, United States., Noh S; Neural Circuit Research Group, Korea Brain Research Institute (KBRI), Daegu 41068, Republic of Korea., Park YJ; College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Seoul 05029, Republic of Korea., Baek SH; Laboratory of Cardiovascular Regeneration, Division of Cardiology, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul 02841, Republic of Korea., Hwang YS; Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea., Shin SR; Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, Massachusetts 02139, United States., Bae H; Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Seoul 05029, Republic of Korea.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2021 Dec 03; Vol. 6 (49), pp. 33511-33522. Date of Electronic Publication: 2021 Dec 03 (Print Publication: 2021).
DOI:	10.1021/acsomega.1c04292
Abstrakt:	Biodegradable cellular and acellular scaffolds have great potential to regenerate damaged tissues or organs by creating a proper extracellular matrix (ECM) capable of recruiting endogenous cells to support cellular ingrowth. However, since hydrogel-based scaffolds normally degrade through surface erosion, cell migration and ingrowth into scaffolds might be inhibited early in the implantation. This could result in insufficient de novo tissue formation in the injured area. To address these challenges, continuous and microsized strand-like networks could be incorporated into scaffolds to guide and recruit endogenous cells in rapid manner. Fabrication of such microarchitectures in scaffolds is often a laborious and time-consuming process and could compromise the structural integrity of the scaffold or impact cell viability. Here, we have developed a fast single-step approach to fabricate colloidal hydrogels, which are made up of randomly packed human serum albumin-based photo-cross-linkable microparticles with continuous internal networks of microscale voids. The human serum albumin conjugated with methacrylic groups were assembled to microsized aggregates for achieving unique porous structures inside the colloidal gels. The albumin hydrogels showed tunable mechanical properties such as elastic modulus, porosity, and biodegradability, providing a suitable ECM for various cells such as cardiomyoblasts and endothelial cells. In addition, the encapsulated cells within the hydrogel showed improved cell retention and increased survivability in vitro. Microporous structures of the colloidal gels can serve as a guide for the infiltration of host cells upon implantation, achieving rapid recruitment of hematopoietic cells and, ultimately, enhancing the tissue regeneration capacity of implanted scaffolds. Competing Interests: The authors declare no competing financial interest. (© 2021 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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