T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma.

Autor:	Sparger EE; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States., Chang H; Center for Companion Animal Health, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States., Chin N; California National Primate Research Center, Department of Medical Microbiology and Immunology, University of California, Davis, Davis, CA, United States., Rebhun RB; Center for Companion Animal Health, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States., Withers SS; Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States., Kieu H; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States., Canter RJ; Surgical Oncology, School of Medicine, University of California, Davis, Sacramento, CA, United States., Monjazeb AM; Radiation Oncology, School of Medicine, University of California, Davis, Sacramento, CA, United States., Kent MS; Center for Companion Animal Health, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2021 Dec 02; Vol. 8, pp. 772932. Date of Electronic Publication: 2021 Dec 02 (Print Publication: 2021).
DOI:	10.3389/fvets.2021.772932
Abstrakt:	Investigation of canine T cell immunophenotypes in canine melanomas as prognostic biomarkers for disease progression or predictive biomarkers for targeted immunotherapeutics remains in preliminary stages. We aimed to examine T cell phenotypes and function in peripheral blood mononuclear cells (PBMC) and baseline tumor samples by flow cytometry, and to compare patient ( n = 11-20) T cell phenotypes with healthy controls dogs ( n = 10-20). CD3, CD4, CD8, CD25, FoxP3, Ki67, granzyme B, and interferon-γ (IFN-γ) were used to classify T cell subsets in resting and mitogen stimulated PBMCs. In a separate patient cohort ( n = 11), T cells were classified using CD3, CD4, CD8, FoxP3, and granzyme B in paired PBMC and single cell suspensions of tumor samples. Analysis of flow cytometric data of individual T cell phenotypes in PBMC revealed specific T cell phenotypes including FoxP3+ and CD25+FoxP3- populations that distinguished patients from healthy controls. Frequencies of IFN-γ+ cells after ConA stimulation identified two different patient phenotypic responses, including a normal/exaggerated IFN-γ response and a lower response suggesting dysfunction. Principle component analysis of selected T cell immunophenotypes also distinguished patients and controls for T cell phenotype and revealed a clustering of patients based on metastasis detected at diagnosis. Findings supported the overall hypothesis that canine melanoma patients display a T cell immunophenotype profile that is unique from healthy pet dogs and will guide future studies designed with larger patient cohorts necessary to further characterize prognostic T cell immunophenotypes. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Sparger, Chang, Chin, Rebhun, Withers, Kieu, Canter, Monjazeb and Kent.)
Databáze:	MEDLINE
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