Mapping novel QTLs for yield related traits from a popular rice hybrid KRH-2 derived doubled haploid (DH) population.

Autor: Kulkarni SR; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Balachandran SM; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Ulaganathan K; Centre for Plant Molecular Biology (CPMB), Osmania University, Hyderabad, 500007 India., Balakrishnan D; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Prasad ASH; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Rekha G; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Kousik MBVN; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Hajira SK; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Kale RR; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Aleena D; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Anila M; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Punniakoti E; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Dilip T; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Pranathi K; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Das MA; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Shaik M; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Chaitra K; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Sinha P; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India., Sundaram RM; Biotechnology Department, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State (TS) 500030 India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2021 Dec; Vol. 11 (12), pp. 513. Date of Electronic Publication: 2021 Nov 29.
DOI: 10.1007/s13205-021-03045-7
Abstrakt: A doubled haploid (DH) population consisting of 125 DHLs derived from the popular rice hybrid, KRH-2 (IR58025A/KMR3R) was utilized for Quantitative Trait Loci (QTL) mapping to identify novel genomic regions associated with yield related traits. A genetic map was constructed with 126 polymorphic SSR and EST derived markers, which were distributed across rice genome. QTL analysis using inclusive composite interval mapping (ICIM) method identified a total of 24 major and minor effect QTLs. Among them, twelve major effect QTLs were identified for days to fifty percent flowering ( qDFF12-1 ), total grain yield/plant ( qYLD3-1 and qYLD6-1 ), test (1,000) grain weight ( qTGW6-1 and qTGW7-1 ), panicle weight ( qPW9-1 ), plant height ( qPH12-1 ), flag leaf length ( qFLL6-1 ), flag leaf width ( qFLW4-1 ), panicle length ( qPL3-1 and qPL6-1 ) and biomass ( qBM4-1 ), explaining 29.95-56.75% of the phenotypic variability with LOD scores range of 2.72-16.51. Chromosomal regions with gene clusters were identified on chromosome 3 for total grain yield/plant ( qYLD3-1 ) and panicle length ( qPL3-1 ) and on chromosome 6 for total grain yield/plant ( qYLD6-1 ), flag leaf length ( qFLL6-1 ) and panicle length ( qPL6-1 ). Majority of the QTLs identified were observed to be co-localized with the previously reported QTL regions. Five novel, major effect QTLs associated with panicle weight ( qPW9-1 ), plant height ( qPH12-1 ), flag leaf width ( qFLW4-1 ), panicle length ( qPL3-1 ) and biomass ( qBM4-1 ) and three novel minor effect QTLs for panicle weight ( qPW3-1 and qPW8-1 ) and fertile grains per panicle ( qFGP5-1 ) were identified. These QTLs can be used in breeding programs aimed to yield improvement after their validation in alternative populations.
Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-03045-7.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest in the publication.
(© King Abdulaziz City for Science and Technology 2021.)
Databáze: MEDLINE
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