Characterization of the first two toxins isolated from the venom of the ancient scorpion Tityus (Archaeotityus) mattogrossensis (Borelli, 1901).

Autor:	de Oliveira NB; Laboratory of Toxinology, Department of Physiological Sciences, Institute of Biology, University of Brasilia (UnB), Brasília, DF, Brazil., Magalhães ACM; Laboratory of Toxinology, Department of Physiological Sciences, Institute of Biology, University of Brasilia (UnB), Brasília, DF, Brazil., Bloch C Jr; EMPRABA Center of Genetic Resources and Biotechnology, Brazilian Agricultural Research Corporation, Brasília, DF, Brazil., Beirão PSL; Department of Biochemistry and Immunology, Institute of Exact Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Silva AO; Department of Biochemistry and Immunology, Institute of Exact Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Melani RD; Laboratory of Toxinology, Department of Physiological Sciences, Institute of Biology, University of Brasilia (UnB), Brasília, DF, Brazil., Barbosa EA; EMPRABA Center of Genetic Resources and Biotechnology, Brazilian Agricultural Research Corporation, Brasília, DF, Brazil.; Laboratory of Biomolecule Synthesis and Analy sis, Institute of Chemistry, University of Brasilia (UnB), Brasília, DF, Brazil., Pires OR; Laboratory of Toxinology, Department of Physiological Sciences, Institute of Biology, University of Brasilia (UnB), Brasília, DF, Brazil., Schwartz CA; Laboratory of Toxinology, Department of Physiological Sciences, Institute of Biology, University of Brasilia (UnB), Brasília, DF, Brazil.
Jazyk:	angličtina
Zdroj:	The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2021 Dec 13; Vol. 27, pp. e20210035. Date of Electronic Publication: 2021 Dec 13 (Print Publication: 2021).
DOI:	10.1590/1678-9199-JVATITD-2021-0035
Abstrakt:	Background: Almost all Tityus characterized toxins are from subgenera Atreus and Tityus , there are only a few data about toxins produced by Archaeotityus , an ancient group in Tityus genus. Methods: Tityus (Archaeotityus) mattogrossensis crude venom was fractionated by high performance liquid chromatography, the major fractions were tested in a frog sciatic nerve single sucrose-gap technique. Two fractions (Tm1 and Tm2) were isolated, partially sequenced by MALDI-TOF/MS and electrophysiological assayed on HEK293 Nav 1.3, HEK293 Nav 1.6, DUM and DRG cells. Results: The sucrose-gap technique showed neurotoxicity in four fractions. One fraction caused a delay of action potential repolarization and other three caused a reduction in amplitude. An electrophysiological assay showed that Tm1 is active on HEK293 Nav 1.3, HEK293 Nav 1.6, DUM and DRG cells, and Tm2 on HEK293 Nav 1.3 and DRG cells, but not in HEK293 Nav 1.6. In addition, Tm1 and Tm2 did promote a shift to more negative potentials strongly suggesting that both are α-NaScTx. Conclusion: Although Tityus (Archaeotityus) mattogrossensis is considered an ancient group in Tityus genus, the primary structure of Tm1 and Tm2 is more related to Tityus subgenus. The patch clamp electrophysiological tests suggest that Tm1 and Tm2 are NaScTx, and also promoted no shift to more negative potentials, strongly suggesting that both are α-NaScTx. This paper aimed to explore and characterize for the first time toxins from the ancient scorpion Tityus (Archaeotityus) mattogrossensis. Competing Interests: Competing interests: The authors declare that they have no competing interests.
Databáze:	MEDLINE
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