Self-Oxygenation of Tissues Orchestrates Full-Thickness Vascularization of Living Implants.

Autor: Farzin A; Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge, MA 02139, USA., Hassan S; Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge, MA 02139, USA., Teixeira LSM; Department of Developmental BioEngineering Technical Medical Centre University of Twente Enschede, The Netherlands., Gurian M; Department of Developmental BioEngineering Technical Medical Centre University of Twente Enschede, The Netherlands., Crispim JF; Department of Developmental BioEngineering Technical Medical CentreUniversity of Twente Enschede, The Netherlands., Manhas V; Biomechanics Research Unit GIGA In Silico Medicine University of Liège Chemin des Chevreuils 1, B52/3, Liège 4000, Belgium., Carlier A; Laboratory for Cell Biology-Inspired Tissue Engineering MERLN Institute University of Maastricht Maastricht, The Netherlands., Bae H; KU Convergence Science and Technology Institute Department of Stem Cell and Regenerative Biotechnology Konkuk University Seoul 05029, Republic of Korea., Geris L; Biomechanics Research Unit GIGA In Silico Medicine University of Liège Chemin des Chevreuils 1, B52/3, Liège 4000, Belgium., Noshadi I; Department of Bioengineering University of California Riverside, CA 92521, USA., Shin SR; Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge, MA 02139, USA., Leijten J; Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge, MA 02139, USA.
Jazyk: angličtina
Zdroj: Advanced functional materials [Adv Funct Mater] 2021 Oct 14; Vol. 31 (42). Date of Electronic Publication: 2021 Jul 06.
DOI: 10.1002/adfm.202100850
Abstrakt: Bioengineering of tissues and organs has the potential to generate functional replacement organs. However, achieving the full-thickness vascularization that is required for long-term survival of living implants has remained a grand challenge, especially for clinically sized implants. During the pre-vascular phase, implanted engineered tissues are forced to metabolically rely on the diffusion of nutrients from adjacent host-tissue, which for larger living implants results in anoxia, cell death, and ultimately implant failure. Here it is reported that this challenge can be addressed by engineering self-oxygenating tissues, which is achieved via the incorporation of hydrophobic oxygen-generating micromaterials into engineered tissues. Self-oxygenation of tissues transforms anoxic stresses into hypoxic stimulation in a homogenous and tissue size-independent manner. The in situ elevation of oxygen tension enables the sustained production of high quantities of angiogenic factors by implanted cells, which are offered a metabolically protected pro-angiogenic microenvironment. Numerical simulations predict that self-oxygenation of living tissues will effectively orchestrate rapid full-thickness vascularization of implanted tissues, which is empirically confirmed via in vivo experimentation. Self-oxygenation of tissues thus represents a novel, effective, and widely applicable strategy to enable the vascularization living implants, which is expected to advance organ transplantation and regenerative medicine applications.
Competing Interests: Conflict of Interest The authors declare no conflict of interest.
Databáze: MEDLINE
Externí odkaz: