Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics.

Autor: Makarova JA; Faculty of Biology and Biotechnology, Higher School of Economics National Research University, 101000 Moscow, Russia., Fomicheva KA; Hertsen Moscow Oncology Research Institute, National Center of Medical Radiological Research, Ministry of Health of the Russian Federation, 125284 Moscow, Russia., Osipyants AI; Hertsen Moscow Oncology Research Institute, National Center of Medical Radiological Research, Ministry of Health of the Russian Federation, 125284 Moscow, Russia.; Far Eastern Federal University, 690091 Vladivostok, Russia., Shkurnikov MY; Hertsen Moscow Oncology Research Institute, National Center of Medical Radiological Research, Ministry of Health of the Russian Federation, 125284 Moscow, Russia., Pokryshchenko AA; Faculty of Biology and Biotechnology, Higher School of Economics National Research University, 101000 Moscow, Russia., Tonevitsky EA; Development Fund, Mendeleev Valley, Innovative Scientific and Technological Center, 125480 Moscow, Russia., Vechorko VI; Filatov City Clinical Hospital no. 15, Department of Health of Moscow, 111539 Moscow, Russia.
Jazyk: angličtina
Zdroj: Applied biochemistry and microbiology [Appl Biochem Microbiol] 2021; Vol. 57 (8), pp. 845-850. Date of Electronic Publication: 2021 Dec 14.
DOI: 10.1134/S0003683821080032
Abstrakt: Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption.
Competing Interests: The authors declare that they have no conflicts of interest.
(© Pleiades Publishing, Inc. 2021, ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 8, pp. 845–850. © Pleiades Publishing, Inc., 2021.Russian Text © The Author(s), 2020, published in Biotekhnologiya, 2020, Vol. 36, No. 5, pp. 6–12.)
Databáze: MEDLINE
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